细胞因子诱导的杀伤细胞治疗胃癌对其无瘤生存的影响

Influence of cytokine-induced killer cell treatment on diease-free suvival of gastric cancer patients

目的探讨体外诱导自体细胞因子诱导的杀伤细胞（CIK）治疗胃癌以无瘤生存为结局的相关因素。方法应用生物技术体外诱导自体细胞因子激活的杀伤细胞进行临床应用。选择1998年1月1Et至2009年12月31日在苏州大学附属第三医院手术治疗并经病理诊断的胃癌病例181例。采用Kaplan．Meier及Log-rank检验比较化疗联合CIK细胞治疗组（CIK治疗组）和化疗组对胃癌无瘤生存的影响。结果对影响预后的重要因素如性别、年龄、胃癌部位、肿瘤大小、组织学类型、浸润深度、淋巴结转移、病理分级等因素进行分析，两组间差异无统计学意义；CIK治疗组胃癌患者的无瘤中位生存时间明显高于化疗组（χ2=9．722，P〈0．01）；且生存时间和CIK细胞治疗次数呈正相关（χ2=14．769，P〈0．01）。结论CIK细胞治疗可以显著延长胃癌患者的中位生存时间。

Objective To study the related factors of autologous cytokine-induced kiUer （CIK） cell treatment in vitro and the disease-free suvival of gastric cancer patients. Methods Gastric cancer patients were treated with autologous cytokine-induced killer cells in vitro by biotechnology. 181 cases of gastric cancer after surgical treatment and pathological diagnosis in the third affiliated hospital of Sooehow University between January 1, 1998 and December 31,2009 were selected in this study. The clinical efficacy of chemotherapy in combination with CIK biotherapy （ CIK treatment group） on the disease-free survival of gastric cancer patients compared to the chemotherapy alone （chemotherapy group） was evaluated by Kap- lan-Meier survival analysis and Log-rank test. Results The difference was not statistically significant in sex, age, tumor location, tumor size, histological types, invasion depth, lymph node metastasis or patho- logical grade. The disease-free median survival time of gastric cancer patients was significantly longer in the CIK treatment group than in the chemotherapy group （χ2 = 9. 722 ,P 〈 0. 01 ）, and there was a positive correlation between the survival time and the times of CIK cell treatment （χ2 = 14. 769, P 〈 0. 01 ）. Conclusion CIK cell treatment can significantly prolong the median survival time of gastric cancer patients.