胃转流术对2型糖尿病大鼠的血糖及胰高血糖素样肽-1的影响

Effect of gastric bypass surgery on fasting blood-glucose and glucagon-like peptide-1 in type 2 dia- betic rats

目的观察胃转流术（GBP）对糖尿病大鼠血糖的控制效果及胰高血糖素样肽-1（GLP-1）的影响。方法采用链脲佐菌素建立糖尿病sD大鼠模型20只，随机分为糖尿病手术组（DO组）和糖尿病对照组（DC组），另取20只非糖尿病大鼠随机分为正常对照组（NC组）和正常手术组（NO组）。分别检测各组大鼠术前、术后72h、1周、4周和8周空腹血糖水平以及血清GLP-1浓度。结果术前DO组与DC组以及NC组与NO组大鼠空腹血糖之间的比较差异均无统计学意义（P〉0．05）；DO组大鼠术后空腹血糖进行性下降，术后8周由术前的（20．84±1．98）mmol／L下降到（5．56±0．11）mmol／L（P〈0．05）；DC组大鼠术前及术后各时相的差异无统计学意义（P〉0．05）。DO组和NO组大鼠术后血清GLP．1浓度出现明显升高（P〈0．05），术后8周分别由术前的（7．10±0．55）、（10．73±0．67）pmol／L上升到（26．48±1．14）、（13．98±0．92）pmol／L（P〈0．05）。结论GBP对2型糖尿病大鼠具有明显的降糖作用，GLP．1的升高在其中起着重要作用，但对正常大鼠血糖无影响。

Objective To investigate the effect of gastric bypass surgery （GBP） on fasting blood- glucose （FBG） and glucagon-like peptide-1 （GLP-1） in type 2 diabetic rats. Methods The models of type 2 diabetic rats were induced by stretozotocin and 20 diabetic rats were randomly divided into two groups： diabetes-operation group （DO group, n = 10） and diabetes-control group （DC group, n = 10）. Another twenty normal rats were randomly divided into two groups： normal-operation （NO group, n = 10） and normal-control group （ NC group, n = 10）. The FBG levels and glucagon-like peptide-1 （ GLP-1 ） con- centrations of rats in each group were detected before operation and 72 h, on 1 week, 4 weeks, 8 weeks af- ter operation. Results The FBG levels of rats before operation were not statistically significant different between DO group and DC group or between NO group and NC group （P 〉 0. 05）. After operation, the FBG levels of rats in DO group gradually declined, eight weeks after operation the FBG levels was declined from （20. 84 ± 1.98） mmol/L to （5. 56± 0. 11 ） mmol/L （P 〈 0. 05） ; In DC group, the difference of FBG levels of rats at different time point was not statistically significant （P 〉 0. 05）. After operation, the con- centrations of serum GLP-1 of rats in DO group and NO group gradually increased, eight weeks after operation The serum GLP-1 elevated respectively from （ 7. 10 ± 0. 55 ）, （ 10. 73 ± 0. 67 ） pmol/L to （ 26. 48 ± 1.14）, （13.98 ±0. 92） pmol/L （P 〈0. 05）. Conclusion There is obvious hypoglyeemic effect of GBP on FBG levels of type 2 diabetic rats other than normal rats, in which high secretion of GLP-1 may be play an important role, high secretion of GLP-1 on the normal type 2 diabetic rats has no hypoglycemie effect.