原发性肝癌发生与ras基因突变的关系

Relationship between primary liver cancer and ras gene mutation

目的探讨H．ras、K-ras、N—ras基因突变与原发性肝癌发生发展的关系。方法采用HotStarTaq聚合酶链反应（PCR）检测32例原发性肝癌组织中H—ras、K—Fas、N-ras基因的表达并采用PCR直接测序分析H—ras、K-ras、N—tab基因突变私检验检测H-ras突变与临床病理的关系。结果在32例原发性肝癌组织中均可检测到H—ras、K-ras、N—ras基因的表达，32例原发性肝癌标本PCR结果经测序后，17例标本发现H-ras基因发生突变（17／32，53．1％），14例40密码子中A突变成G，3例62密码子中G突变成A。K—ras、N-ras基因未发生突变，H-ras突变与肿瘤转移有关。结论H-ras基因异常表达与肝癌相关，可能导致了原发性肝癌的发生。

Objective To investigate the association between mutation of H-ras, K-ras, N-ras and carcinogenesis of primary liver cancer （PLC）. Methods HotStarTaq polymerase chain reaction （PCR） was used to detect the expression of H-ras, K-ras, N-ras gene in 32 PLC patientds and the mutation of H- ras, K-ras, N-ras gene was analyzed by PCR and direct sequencing. Results The expression of H-ras, K- ras, N-ras was detected in 32 cases. Sequencing indicates that H-ras mutation occur in 17 cases, among which 14 cases was verified to be a A/G transition in codon 40 and 3 cases G/A transition in codon 62. K- ras, N-ras had no mutation. H-ras mutation was correlated to tumor metastasis. Conclusion Abnormal expression of H-ras is correlated with PLC and may lead to the the carcinogenesis of PLC.