黑色素瘤抗原-D4在非小细胞肺癌组织中的表达及其意义

Expression and significance of melanoma antigen melanoma antigen-D4 in non-small cell lung caner

目的检测黑色素瘤抗原（MAGE）-D4基因在非小细胞肺癌（NSCLC）组织及正常组织中mRNA的表达水平差异。方法对54例NSCLC患者按年龄、性别、吸烟史、肿瘤大小、病理类型、细胞分化程度、淋巴结有否转移等进行分组，并检测其MAGE-D4的表达。结果MAGE-D4在NSCLC组织的表达（1．74）高于其在正常组织中的表达（0．55，P〈0．05）。MAGE-D4的表达与患者年龄、性别、烟龄、肿瘤大小无关；其在鳞癌中的表达（1．99）高于腺癌（1．27，P〈0．05）；在细胞分化差的组织中表达（2．96）高于细胞分化较好的组织（1．01，P〈0．05）；在淋巴结转移者中的表达（2．07）高于无淋巴结转移者（1．42，P〈0．05）。结论MAGE-134基因在NSCLC组织中有较高的表达水平，并存在一定的表达规律，可作为NSCLC的特异性抗原。

Objective To study the differential expression of melanoma antigen （MAGE）-134 gene in non-small cell lung caner （NSCLC） and normal lung tissue. Methods Fifty-four NSCI~ patients were classified by age, sex, smoke history, tumor size, pathologic type, cytodifferentiation, lymphatic metas- tasis. MAGE-D4 gene expression patterns were detected in NSCLC. Results The expression of MAGE-D4 gene in NSCLC was higher than that in normal lung tissue （ 1.74 vs 0. 55 ,P 〈0. 05）. The expression level has no relationship with age, sex, smoke history and tumor size. The squamous cell carcinoma had a higer expression than adenocacinoma （ 1.99 vs 1.27, P 〈 0. 05）. The expression in poorly differentiated tumor was higher than that in moderately differentiated tumor （ 2. 96 vs 1.01 P 〈 0. 05 ）. The expression in NSCLC with lymphatic metastasis was higher than in that without lymphatic metastasis （2. 07 vs 1.42, P 〈 0. 05） . Conclusion MAGE-IM gene has a higher expression level in NSCLC and its expression may follow a certain rule. The MAGE-D4 gene could be potentially a specific NSCLC antigen.