一株踝节霉菌的培养特性及其在污泥生物淋滤中的应用

Cultural Characteristics of a Strain of Talaromyces sp.and Its Application in Bioleaching of Sewage Sludge

于实验室嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)淋滤处理城市污泥酸性废液中分离出一株踝节霉菌ZJ-1,并对其进行了摇瓶液体培养特性研究.单因素试验结果表明:该菌株的最适碳源和氮源分别为葡萄糖和酵母膏,最适温度为28℃,最适转速为140 r/min,最适初始pH值为5.0.正交试验优化得到碳、氮源的最优水平分别为葡萄糖70 g/L,酵母膏15 g/L.采用HPLC对该菌株PDA培养液中的有机酸进行了分析,发现其中含有丙酮酸.初始PDA培养液中丙酮酸含量为0.039 g/L,接菌培养d 5.5时其含量达0.106 g/L.为探讨踝节霉菌ZJ-1对嗜酸氧化亚铁硫杆菌淋滤污泥过程的影响,在生物淋滤起始阶段接种10%A.ferrooxidans和4%踝节霉菌ZJ-1,以仅接种10%A.ferrooxidans作为对照.结果表明A.ferrooxidans和踝节霉菌ZJ-1的复合菌组与对照组相比,淋滤耗时由7 d缩短至4 d,淋滤处理时间缩短了43%.

A strain,named as Talaromyces sp.ZJ-1,was isolated from the acid wastewater of sewage sludge with Acidithiobacillus ferrooxidans bioleaching.The liquid fermentation conditions of the strain were studied in rotary flask.The results of the single factor analysis showed that the optimal carbon and nitrogen sources were glucose and yeast extract,respectively.The optimal fermentation temperature,rotation rate and initial pH were 28 ℃,140 r/min and 5.0,respectively.Orthogonal test indicated that the optimal concentrations of carbon and nitrogen sources were 70 g/L glucose and 15 g/L yeast extract,respectively.HPLC analysis was used to determine organic acids in PDA broth of the strain,and pyruvate was detected with the initial concentrations 0.039 g/L and it reached 0.106 g/L after 5.5 days of culturing.To examine the effect of Talaromyces sp.ZJ-1 on sewage sludge bioleaching,the bioleaching tests of sewage sludge were carried out by inoculating 10% A.ferrooxidans and 4% Talaromyces sp.ZJ-1,and only 10% A.ferrooxidans as control.The results revealed that the required period of bioleaching was shortened from 7 d to 4 d,with 43% less than that of the control.