组蛋白去乙酰化酶抑制剂MS-275对乳腺癌MCF7细胞周期作用及分子机制探讨

Effect of histone deacetylase inhibitor MS - 275 to breast cancer MCF7 mitotic cycle and its molecular mechanism

目的 研究组蛋白去乙酰化酶抑制剂MS-275对乳腺癌MCF7细胞周期的作用及机制.方法 用0.5、1.0、1.5;μmol/L MS-275作用于乳腺癌MCF7细胞24 h,用MTT 法观察不同浓度MS-275对乳腺癌MCF7细胞的生长抑制作用,流式细胞仪检测乳腺癌MCF7细胞生长及周期,Western-blotting法检测MS-275对乳腺癌MCF7细胞周期相关基因表达的影响.结果 1.0 μmol/L的MS-275对乳腺癌MCF7细胞有明显的生长抑制作用,并呈现一定的量效关系,在1.0 μxmol/L浓度之上可明显诱导乳腺癌MCF7细胞周期阻滞,增强p21、p27基因的表达,降低CCNDl、Cdk4D基因的表达.结论 一定剂量MS-275抑制乳腺癌MCF7细胞的生长,细胞阻滞的发生与p21、p27基因表达的增加及CCND1、Cdk4D基因表达的减少相关.

Objective To study the effect of histonedeacetylase inhibitor MS - 275 to the breast cancer MCF7 cell cycle and the mechanism. Methods 0.5, 1.0, 1.5 punol / L MS - 275 affects the MCF7 cell for 24 hours, observes different density MS -275 with MTT to the MCF7 cell＇s growth inhibitory action; flow eytometry examination cell growth and the cycle; Western- blotting examined MS -275 to the mammary gland cancer cell cycle related gene expression. Results 1.0 mol/L density MS -275 had the obvious growth inhibitory effect to the MCF7 cell, and presented certain dose effect relationship, may induce the breast cancer cell MCF7 mitotic cycle above the 1.0 mol/L density to hinder ob- viously, may strengthen the p21, p27 gene obviously the expression, reduced CCND1, the Cdk4D gene expression obviously. Conclusion Certain dosage＇s MS -275 suppresses breast cancer MCF7 cell＇s growth, may through regulate many mitotic cycle related gene induction mammary gland cancer cell mitotic cycle to hinder, the cell hinders occurrence and p21, p27 gene expression increase and CCND1, Cdk4D gene expression ,reduced related.