慢性间断性缺氧对帕金森病模型小鼠黑质凋亡相关蛋白Bcl-2和Bax表达的影响

Effect of chronic intermittent hypoxia on apoptosis-related protein Bcl-2 and Bax in substantia nigra of parkinson's disease model mouse

目的探讨慢性间断性缺氧(CIH)对帕金森病模型小鼠黑质凋亡相关蛋白Bcl-2和Bax表达的影响。方法 44只雄性6周龄C57BL/6小鼠,随机分为对照组、CIH组、百草枯组和百草枯+CIH组,应用免疫组织化学方法(SP法)检测小鼠中脑黑质Bax和Bcl-2蛋白的表达。结果对照组、CIH组、百草枯组、百草枯+CIH组中脑黑质Bcl-2阳性细胞计数分别为39.45±1.51、28.64±2.66、21.64±1.85、8.45±1.86,Bcl-2的表达逐渐降低,CIH+PQ组较其它3组明显降低(P<0.01);中脑黑质Bax的阳性细胞计数分别为11.91±1.70、20.73±1.49、30.09±2.98、52.82±2.86,Bax的表达逐渐升高,CIH+PQ组较其它3组明显升高(P<0.01)。结论慢性间断性缺氧联合百草枯暴露加重了小鼠黑质多巴胺能神经元凋亡相关蛋白Bax的上调及Bcl-2表达的下调。

[Objective]To study the effect of chronic intermittent hypoxia（CIH） on the expression of Bcl-2 and Bax proteins in substantia nigra of paraquat（PQ） parkinson＇s disease model mouse.[Methods]Forty-four six-week -old male C57BL/6 mice were randomly divided into four groups：control group,CIH group,PQ group and PQ＋ CIH group.The expression of Bcl-2 and Bax proteins in substantia nigra of the mice were detected by S-P immunohistory chemistry staining.[Results]The positive cell number of Bcl-2 in nigra were 39.45±1.51,28.64±2.66, 21.64±1.85,8.45±1.86 in the control group,CIH group,PQ group and PQ＋CIH group.The expression of Bcl-2 was reduced gradually in four groups,the reduction of PQ＋CIH group was most apparent in the among group（P〈0.01）; The positive cell number of Bax in nigra were 11.91±1.70,20.73±1.49,30.09±2.98,52.82±2.86 in the control group, CIH group,PQ group and PQ＋CIH group,the expression of Bax was increased gradually and the increase of PQ ＋ CIH group was most apparent in the among group（P〈0.01）.[Conclusion]CIH combined with paraquat can upregulate the expression of Bax protein and downregulate the expression of Bcl-2 protein of dopaminergic neuron in the nigra in C57BL/6 mice.