Id-1在宫颈癌组织中的表达及其临床意义

The expression and clinical significance of Id-1 in cervical cancer

目的检测细胞分化抑制因子-1(Id-1)mRNA和蛋白在人宫颈癌组织中的表达,探讨Id-1的表达意义。方法分别用荧光实时定量逆转录-聚合酶链反应(RT-PCR.)和免疫组织化学法,检测Id-1 mRNA和蛋白在人正常宫颈、宫颈上皮内瘤变和宫颈癌组织中的相对表达定量,分析Id-1变化与宫颈癌病理参数间的相关性。结果 Id-1 mRNA和蛋白在正常宫颈组织中均无表达,在宫颈上皮内瘤变组织中有少量表达,在宫颈癌组织中的含量明显升高。Id-1 mRNA和蛋白表达在宫颈癌中呈直线正相关。Id-1高表达和癌组织分化呈负相关,和FIGO分期呈正相关。结论宫颈癌组织中Id-1表达明显升高,而且与癌组织病理分级和临床分期相关。Id-1检测有助于对宫颈癌进行早期辅助诊断。

[ Objective] To investigate the mRNA and protein expression of Id-1 （inhibitor of differentiation-1 or inhibitor of DNA binding-1） in human cervical cancer and analysis its clinical significance. [Methods] The quantity of Id-1 mRNA was detected by real time RT-PCR, and the protein expression was detected by immunohistochemical method in normal cervical samples, cervical intraepithelial neoplasias （CIN） and cervical cancers. The rela- tionship between Id-1 and some clinical parameters was further analyzed. [Results ] No Id-I mRNA and protein was shown in normal cervical tissues. Lower expression of Id-1 mRNA and protein was detected in CIN. Higher ex- pression of Id-1 mRNA and protein was shown in cervical cancers. The expression of Id-1 mRNA and protein was positive correlation in cervical cancers. Moreover, higher expression of Id-1 was negative correlated with the differentiation, and positive correlated with the FIGO stage in ovarian cancers. [ Conclusion ] The expression of Id-1 is significantly increased in cervical cancer, which is obviously correlated with the pathological grade and clinical stage in cervical cancer. The detection of Id-1 can help the diagnosis and prognosis of cervical cancer.