慢性肝病和原发性肝细胞癌组织中MCM2和TIP30的表达及意义

Expressions of MCM2 and TIP30 and their clinicopathological significances in the primary hepatocellular carcinoma(HCC) and chronic liver diseases(CLDs)

目的研究肝细胞癌（HCC）、癌旁组织和慢性肝病（CLDs）组织中MCM2和TIP30表达水平及其临床病理意义。方法50例HCC、25例癌旁组织手术切除标本及66例CLDs穿刺标本常规制作石蜡包埋切片，MCM2和TIP30染色方法为EnVision免疫组化法。结果HCC中MCM2表达阳性率明显高于除肝硬化外的各类型CLDs和癌旁组织（P〈0．05或P〈O．01）；HCC中TIP30表达阳性率明显低于癌旁组织及轻、中度CHB（P〈0．05或P〈0．01）；癌旁组织和慢性肝病中MCM2表达阳性率随G分级和S分期的增高而增高，但TIP30表达阳性率随G分级和s分期的增高而下降（P〈0．05或P〈0．01）。高＋中分化、肿块最大径≤5cm、无肝硬化、无肝内外转移及无癌栓病例MCM2表达阳性率明显低于低分化、肿块最大径〉5cm、肝硬化、肝内外转移及癌栓病例（P〈0．05）；但TIP30表达阳性率则相反（P〈0．05）；HCC中MCM2和TIP30表达呈明显不一致性（χ2=8．12，P〈0．01）。结论MCM2和TIP30表达水平与HCC发生、进展及临床生物学行为密切相关，且两者表达水平与癌旁组织及CLDs中炎性细胞浸润分级、纤维化分期及肝硬化发生密切相关。

[ Objective ] To study the expression levels of MCM2 and TIP30 and detect their clinieopathological signifieanees in the HCC, peritumoral tissues and CLDs. [ Methods ] EnVision immunohistoehemical method for determining the expressions of MCM2 and TIP30 was used in routinely paraffin-embedded sections of surgical reseeted specimens from HCC （n =50） and its peritumoral tissues （n =25）, and punetural specimens of CLDs （n =66）. [Results ] The positive rate of MCM2 expression in HCC was significantly higher than that in peritumoral tissues and CLDs （P 〈0.05 or P 〈0.01）, but the positive rate of TIP30 expression was significandy lower in HCC than that in pefitumoral tissues and mild or middle CHB （P 〈0.05 or P 〈0.01）. The increased positive rates of MCM2 expression were associated with increased grades of inflammative infifiation （G） and stages of fibrosis （S） in CLDs. The increased positive rates of TIP30 expression were associated with decreased grades of inllammative infifiation （G） and stages of fibrosis （S） in CLDs. The positive rates of MCM2 were significandy lower in the cases of well-＋moderately-differen- tiated adenocareinoma, maximal diameter of mass 〈5 em, no-cirrhosis, no-metastasis of intra- and extra-liver and no-cancer embolus than those in the ones of poorly-differentiated adenoeareinoma, maximal diameter of mass 〉5 em, eirrhosis, metastasis of intra- and extra-liver, and embolus of cancer cells in HCC （P 〈0.05）. The positive rates of TIP30 were significantly higher in the cases of well-＋moderately-differentiated adenocareinoma, maximal diameter of mass 〈5 cm, no-cirrhosis, no-metastasis of intra- and extra-liver, and no-embolus of cancer cells than those in the ones of poorly-differentiated adenocarcinoma, maximal diameter of mass 〉5 cm, crirrhosis, metastasis of intra- and extra-liver, and embolus of cancer cells in HCC （P 〈0.05）. The inconsistency was found between the expression of MCM2 and TIP30 in HCC （χ2=8.12, P 〈0.01）. [Conclusion] The expression of MCM2 and/or TIP30 may be closely related to the carcinogenesis, clinical biological behaviors, and prognosis of HCC. And the expression of MCM2 and/or TIP30 may be closely related to infiliation of inflammative cells, progression of fibrosis, and pathogenesis of cirrhosis in CLDs.