朱顶红体细胞胚胎发生及植株再生研究

Somatic Embryogenesis and Plant Regeneration in Hippeastrum hybridum

通过诱导朱顶红(Hippeastrum hybridum)小鳞茎形成胚性愈伤组织,建立间接的体细胞胚胎发生途径和植株再生的无性繁殖体系。试验结果表明,0.5mg.L-1的2,4-D附加0.5mg.L-16-BA对试管小鳞茎外植体初代胚性愈伤组织的诱导和继代增殖有明显的促进作用,初培养松脆愈伤组织诱导率可达83.3%。2.5～5.0mg.L-1的6-BA对体细胞胚胎的发育有显著的促进作用,培养50d大于20个体细胞胚发生的愈伤组织约为50%;相同浓度6-BA附加不同浓度NAA的试验组间体细胞胚发育无明显差异。

An effective micropropagation system via callus-mediated（indirect）somatic embryogenesis was established,using in vitro bulblets as explants in Hippeastrum hybridum.The results showed that 2,4-D（0.5 mg·L1）and 6-BA（0.5 mg·L-1）had important role in inducing embryogenic callus from in vitro bulblets and in multiplication of callus during sub-cultures.The percentage of friable callus formation was 83.3%in primary culture.The 6-BA（2.5-5.0 mg·L1）had a notable effect on development of somatic embryos.The rate of callus with more than twenty somatic embryos was about 50%for 50 days.Development of somatic embryos had no difference on the same 6-BA media supplemented with different concentrations of NAA.