红细胞生成素抑制C3a介导的肾小管上皮细胞间充质转分化

Erythropoietin inhibits complement 3a-induced renal tubular epithelial to mesenchymal transition

目的 观察红细胞生成素（EPO）对补体成分C3片段C3a介导的肾小管上皮细胞间充质转分化（EMT）的影响.方法 将人近端肾小管上皮细胞（HK-2）分为6组：对照组、EPO组、TGF-β组、TGF-β＋EPO组、C3a组、EPO＋C3a组,分别用RT-PCR、Western印迹和细胞免疫荧光方法检测HK-2细胞α-SMA、E-cadherin、C3的mRNA和蛋白表达.结果 与对照组和EPO组比较,C3a或TGF-β干预HK2细胞后,αt-SMA mRNA和蛋白表达增强（均P＜0.05）,E-cadherin mRNA和蛋白表达减少（均P＜ 0.05）,补体C3 mRNA和蛋白表达增强（均P＜ 0.05）；而同时给予EPO干预后,C3a或TGF-β的上述作用可被明显减弱（均P＜0.05）.结论 EPO可抑制C3a介导的肾小管上皮细胞EMT.

Objective To investigate the effects of erythropoietin （EPO） on complement 3a （C3a）-induced renal tubular epithelial to mesenchymal transition. Methods The HK-2 cells were divided into 6 groups namely control group,EPO group,TGF-β group,TGF-β＋EPO group,C3a group and EPO＋C3a group.The mRNA and protein expressions of α-SMA,E-cadherin and C3 were investigated by RT-PCR,Western blot and immunofluorescence respectively. Results Compared with control group and EPO group,the mRNA and protein expressions of α-SMA in HK-2 cells were up-regulated after the intervention of C3a or TGF-β （all P〈0.05）.On the contrast,the mRNA and protein expressions of E-cadherin were down-regulated（P〈0.05）,the mRNA and protein expressions of C3 were enhanced （all P〈0.05）.However,all those above effects of C3a or TGF-β were inhibited after the intervention of EPO （all P〈0.05）. Conclusion EPO is capable of suppressing the epithelial to mesenchymal transition induced by C3a.