血管内皮生长因子对大鼠卵巢组织自体移植后的形态与功能的影响

Effect of VEGF on morphology and function of rat ovarian tissues after autologous transplantation

目的:初步探讨血管内皮生长因子(VEGF)在卵巢组织自体移植中的应用价值。方法:将健康成熟未交配过的20只雌性SD大鼠随机分为2组,A组去势后自体异位移植新鲜卵巢组织,B组去势后自体异位移植经VEGF处理过的新鲜卵巢组织。移植后饲养4周,观察动情周期的恢复情况和卵巢组织的形态学变化,测定血清中的激素水平。结果:B组大鼠的雌二醇水平明显高于A组,其差异有统计学意义(P<0.05);B组移植卵巢组织的体积明显大于A组,其差异有统计学意义(P<0.05);对形态正常的各级卵泡统计分析,生长卵泡数目在各组之间的差异没有统计学意义。结论:在卵巢组织移植过程中使用VEGF,可以减少卵巢组织的缺血再灌注损伤,有助于促进卵巢组织功能的恢复。

AIM： To evaluate the effect of vascular endothelial growth factor（VEGF） on the ovarian tissues during transplantation.METHODS： Twenty regularly cycling rats were randomly divided into 2 groups： the rats in group A received fresh autologous ovarian transplantation without VEGF administration（n=10）;the rats in group B received fresh autologous ovarian transplantation with VEGF administration（n=10）.Four weeks after transplantation,the ovarian tissues and sera of the rats were collected for histological examination and determination of the hormone levels.RESULTS： The serum concentration of estradiol in group B was significantly higher than that in group A（P0.05）.Four weeks after transplantation,we found that the ovarian tissues were significantly developed,and the average size of the ovaries in group B was significantly larger than that in group A（P0.05）.CONCLUSION： The ovarian tissues with VEGF administration develop better than those without VEGF administration.VEGF can increase the graft vascularity and the percentage of surviving tissues.