糖尿病小鼠肾小球微血管密度与VEGF表达的研究

Microvessel density and expression of vascular endothelial growth factor in glomeruli of diabetic mice

目的:探讨糖尿病小鼠肾小球微血管密度(MVD)变化及其与血管内皮生长因子(VEGF)表达的关系。方法:链脲佐菌素诱导小鼠糖尿病6周,定期测量对照组与模型组小鼠体重与血糖变化;常规HE染色观察肾脏形态学变化,采用组织细胞病理分析软件测量肾小球直径、周长及面积;应用免疫组织化学方法检测CD34及VEGF在肾小球的表达,计算MVD值及VEGF表达指数。结果:与对照组相比,模型组小鼠血糖明显升高(P<0.01),体重显著降低(P<0.01),肾小球直径、周长及面积明显增大(P<0.05);肾小球CD34及VEGF表达明显增强(P<0.01),肾小球MVD显著增高且与VEGF表达呈显著正相关(r=0.9979,P<0.05)。结论:VEGF可促进糖尿病小鼠肾小球新生血管生成,VEGF表达上调与糖尿病肾脏病变密切相关。

AIM： To investigate the relationship between microvessel density（MVD） and expression of vascular endothelial growth factor（VEGF） in glomeruli of diabetic mice.METHODS： Streptozotocin-induced diabetic mice as well as the control mice were involved in this study for 6 weeks.The body weight and blood glucose level of the mice in each group were weekly measured at certain time point.The morphological changes of the kidney were observed under light microscope,and the diameter,perimeter and area of the glomeruli were detected by an image analysis system.The expression of CD34 and VEGF in glomeruli was examined by immunohistochemistry method,and MVD and VEGF index were also calculated.RESULTS： In comparison with the control mice,the blood glucose level was significantly increased,and the body weight was decreased in diabetic mice（P0.01）.The diameter,perimeter and area of glomeruli in diabetic mice were significant greater than those in control mice（P0.05）.Increased expression of CD34 and VEGF in the glomeruli of diabetic mice was observed.Glomerular MVD of diabetic mice was significantly higher than that of the controls（P0.01）,and was positively correlated with the VEGF index（r=0.9979,P0.05）.CONCLUSION： VEGF may promote the angiogenesis in glomeruli of diabetic mice.The increase in VEGF expression may play a role in the pathogenesis of diabetic nephropathy.