人羊膜诱导人骨髓间充质干细胞向表皮样细胞的分化

Differentiation of human bone marrow derived mesenchymal stem cells into epidermal-like cells induced by human amniotic membrane

背景:国内外的研究多采用细胞因子诱导骨髓间充质干细胞分化为表皮样细胞,而较少用人羊膜与骨髓间充质干细胞共培养的方法进行诱导分化。目的:观察人羊膜诱导人骨髓间充质干细胞向表皮样细胞分化的可行性。方法:体外分离培养、扩增人骨髓间充质干细胞,选用第5代人骨髓间充质干细胞与健康人羊膜共培养12d进行诱导分化。结果与结论:人骨髓间充质干细胞贴壁生长,呈长梭形,流式细胞仪检测表达CD29,CD44的阳性细胞数分别为96.6%,94.6%。人羊膜与人骨髓间充质干细胞共培养12d,人骨髓间充质干细胞渐变为扁平的表皮样细胞,免疫组化染色显示CK19散在阳性,广谱CK为弥漫阳性。说明人羊膜可诱导人骨髓间充质干细胞分化为表皮样细胞。

BACKGROUND： Cytokines are mostly used for inducing the differentiation of bone marrow mesenchymal stem cells （BMSCs） into epidermal-like cells, and human amniotic membrane is less applied to induce the differentiation of BMSCs through co-culture method. OBJECTIVE： To explore the feasibility of human BMSCs into epidermal-like cells by using human amnion membrane. METHODS： Human BMSCs were isolated, cultured, expanded and identified by cell morphology and expression of CD29, CD44 with flow cytometre. The 5th passage BMSCs were co-cultured with human amniotic membrane for 12 days. RESULTS AND CONCLUSION： Human BMSCs displayed a fibroblast-like morphology and the positive cells of CD29 and CD44 were 96.6% and 94.6%, respectively. After co-culture with human amnion for 12 days, the BMSCs differentiated into epidermal-like cells with diffuse Pan-CK, focal CK19. These findings indicate that human amnion can induce human BMSCs to differentiate into epidermal-like cells