脐血间充质干细胞移植脊髓损伤恢复期患者的免疫功能变化

Effect of umbilical cord blood mesenchymal stem cells transplantation on immune function of spinal cord injury patients

背景:间充质干细胞移植因其具有一定的免疫原性,对脊髓损伤患者免疫功能的影响尚无深入系统的报道。目的:观察脐血源间充质干细胞移植对脊髓损伤患者免疫功能的影响。方法:61例脊髓损伤患者采用静脉滴注结合腰椎脊髓蛛网膜下腔注射脐血间充质干细胞悬液治疗。应用流式细胞术及免疫比浊法分别测定患者移植前后T淋巴细胞亚群、免疫球蛋白及补体的含量。结果与结论:与治疗前相比,患者CD3+、IgA及IgG有下降趋势,差异有显著性意义;CD4+、CD8+、CD4+/CD8+、IgM、C3及C4均有升高或下降趋势,但差异无显著性意义。提示脐血源间充质干细胞移植对细胞及体液免疫不会激活急性免疫应答,移植是安全的,存在负向免疫调节作用的可能,但尚需进一步完善相关指标,扩大样本,明确相关机制。

BACKGROUND： Mesenchymal stem cells （MSCs） transplantation has certain immunogenicity. However, there was no in-depth and system reports about the effect of MSCs transplantation on spinal cord injury patient’s immune function. OBJECTIVE： To observe the effects of umbilical cord blood MSCs （UCB-MSCs） transplantation on immune function of spinal cord injury （SCI） patients.METHODS： Sixty-one spinal cord injury （SCI） patients were treated by intervenous drop infusion and lumbar subarachnoid space puncture to UCB-MSCs. Flow-cytometry and immunoturbidimetry （ITM） were used to detect the content of T lymphocytic subgroup and immune globulin and the alexinic in plasma of all the patients prior and post-transplantation. RESULTS AND CONCLUSION： Compare with pre-transplantation, the content of CD3＋, IgA and IgG was decreased and the differences were significant; The content of CD4＋, CD8＋, CD4＋/CD8＋, IgM, C3 and C4 was also changed and the differences were not significant. It indicates that UCB-MSCs transplantation could not activate the immunological response of cell and humoral immunity, so the transplantation is safety. Negative accommodation is exist, but the relevant indicators need a further improvement, expand samples, and clearly relevant mechanism.