骨关节炎兔软骨细胞凋亡与八味柔肝散的抑制效应

Inhibitory effects of Bawei Rougan powder on chondrocytes apoptosis in rabbits with osteoarthritis

背景:传统中药对骨关节炎具有镇痛等治疗作用,但对关节软骨是否有保护作用及其通过何种机制起作用少有报道。目的:观察八味柔肝散对兔骨关节炎软骨细胞凋亡的影响。方法:将新西兰大白兔随机分为正常组、模型组和治疗组,模型组和治疗组行Hurth法骨关节炎造模。建模后1周,正常组和模型组每天生理盐水灌胃,治疗组行八味柔肝散水提液灌胃。建模后8周行组织学观察Mankin's评分,硝酸还原酶法测定关节液一氧化氮、诱导型一氧化氮合酶活性,原位末端转移酶标记技术检测软骨细胞的凋亡指数。结果与结论:结果显示,治疗组关节软骨Mankin's评分,关节液一氧化氮、诱导型一氧化氮合酶活性,关节软骨细胞凋亡指数均低于模型组(P<0.05)。证实八味柔肝散对兔骨关节炎软骨细胞凋亡有抑制作用。

BACKGROUND： Traditional Chinese Medicine （TCM） has analgesic effects on osteoarthritis, but whether TCM can protect articular cartilage and through which mechanism are rarely reported. OBJECTIVE： To observe the effects of Bawei Rougan powder on chondrocytes apoptosis in rabbits with osteoarthritis. METHODS： New Zealand adult white rabbits were randomly divided into three groups： normal, model, and treatment groups. Osteoarthritis models were established in model and treatment groups according to Hurth technique. At 1 week after modeling, rabbits in the normal and model groups were treated with saline solution, and the treatment group was treated with the powder extract. At 8 weeks after modeling, characteristics of cartilage histological were observed by Mankin method. Activity of nitric oxide （NO） and inducible nitric oxide synthase （iNOS） in synovial fluids were measured by nitrate reductase assay, and apoptosis index of chondrocytes in all groups were measured by TUNEL （TdT-mediated biotinylated-dUTP nick end labeling method）. RESULTS AND CONCLUSION： Compared with the model group, Mankin＇s score, activity of NO and iNOS and apoptosis index of chondrocytes were decreased in the treatment group （P 0.05）. It is confirmed that Bawei Rougan powder can suppress the apoptosis of chondrocytes in rabbits with osteoarthritis.