microRNA对角质形成细胞功能的影响

MicroRNA influences the function of keratinocytes

背景:以角质形成细胞为基础的再上皮化过程是皮肤创面得以顺利修复的关键环节之一。有研究报道在小鼠缺血性创面中microRNA-210负向调控角质形成细胞的增殖,阻碍创面再上皮化的进行,提示microRNA可能通过影响角质形成细胞的生物学活动,进而参与创面修复过程。目的:全面了解microRNA对角质形成细胞生物学活动的影响,指导创面愈合研究领域研究方向的选择,以及为异常创面修复的预防和治疗提供理论依据。方法:以"keratinocyte,microRNA"为检索词检索PubMed,Embase数据库(2011-05),语言限定为英文。共收集文献59篇,阅读题目和摘要进行初筛,排除研究方向与本文无关、内容重复性研究,共保留12篇文章。对所保留文章的参考文献进行手工检索后,另添加文献30篇以及microRNA数据库2个。结果与结论:microRNA对角质形成细胞的增殖、分化和移行能力具有调控作用,特别在缺血性创面中对再上皮化有阻碍作用,有望成为一种潜在的治疗靶点。但目前大部分研究仍为体外实验,需要将现有发现向动物模型以及临床研究转化。

BACKGROUND： The re-epithelialization process based on keratinocyte is essential for the regeneration of wound stratified epithelium. It is reported that microRNA-210 can negatively regulate the keratinocyte proliferation in murine ischemic wound model, and can block the re-epithelialization process of the wound. It indicates that microRNAs may involve in wound repair by influencing the biological activities of keratinocyte. OBJECTIVE： To thoroughly understand the effects of microRNAs on the biological activities of keratinocyte; to direct the research strategies on wound healing; and to provide theoretical basis for the prevention and treatment of abnormal wound repair. METHODS： An online search of PubMed and Embase database （2011-05） was performed using Keywords of ＂keratinocyte＂, ＂microRNA＂ for articles published in English. A total of 59 papers were collected. The irrelevant or repetitive papers were eliminated by screening for the titles and abstracts. A number of 12 papers were retained. These papers were manually retrieved in addition with 30 additional papers and 2 microRNA databases. RESULTS AND CONCLUSION： MicroRNA can regulate the proliferation, differentiation and migration of keratinocyte. In particularly, it can block the re-epithelialization process of ischemic wound. It is expected to be a possible therapeutic target. But most of the present research is focused on in vitro experiment. It is necessary that the existed findings can be applied in animal model and clinical research.