湖羊不同发育阶段卵泡内代谢产物和激素含量的比较研究

A Comparative Study of Metabolites and Hormone Concentrations in Different-sized Follicles in Hu Sheep

本试验旨在研究代谢产物、代谢激素和生殖激素在湖羊黄体期不同发育卵泡内的变化。选用体质量40kg左右的湖羊11头,同期发情结束后第12天屠宰,按不同大小卵泡分离卵泡液。试验结果表明,与≤2.5mm卵泡相比,>2.5mm卵泡内的葡萄糖浓度显著提高(P<0.05),胰高血糖素浓度显著降低(P<0.05),乳酸脱氢酶(LDH)活性和睾酮浓度极显著降低(P<0.01),雌二醇浓度极显著提高(P<0.01),而血氨、游离脂肪酸、尿素、胰岛素和孕酮浓度差异不显著。雌二醇浓度与LDH活性呈极显著负相关(P<0.01),与葡萄糖浓度呈显著正相关(P<0.05),与胰高血糖素浓度呈显著负相关(P<0.05),与睾酮浓度呈极显著负相关(P<0.01),与孕酮浓度接近正相关(P=0.051)。试验结果表明代谢产物和激素共同参与调节卵泡发育。

This study was conducted to investigate the metabolites, metabolm hormones ana reproductive hormones concentrations in different-sized follicles in Hu sheep during the luteal phase. 11 multiparity Hu sheep with 40 kg in body weight were used. On the 12th day after estrus synchronization, all ewes were slaughtered and follicular fluid was collected according to different-sized follicles. The effects of follicle size on serum ammonia, NEFA, urea, insulin and progesterone concentrations were not significant. Compared with follicles ≤2.5 mm in diameter, the follicular fluid glucose and estradiol concentrations in follicles 〉2. 5 mm in diameter were increased （P〈0.05）, and the testosterone and glucagon concentrations and LDH activity were decreased （P〈0.05）. The estradiol concentration in follicular fluid was positively correlated with glucose （P〈0.05） and progesterone （P = 0. 051） concentration and negatively correlated with LDH （P〈0.01）, glucagon （P〈0.05） and testosterone （P〈0.01） concentration. In conclusion, follicle development was co-regulated by intrafollicular metabolites and hormones.