粒细胞集落刺激因子及其受体在儿童肾母细胞瘤中的表达

Expression of G-CSF and its receptor in children Wilm's tumor

目的检测粒细胞集落刺激因子(granulocyte colony-stimulating factor,G-CSF)及其受体(G-CSFR)在儿童肾母细胞瘤中的表达。方法收集我院2009年1月至2011年6月确诊的肾母细胞瘤患者手术标本27例。应用免疫组织化学方法检测肿瘤标本的G-CSF、G-CSFR的表达和分布情况。结果肾母细胞瘤标本中粒细胞集落刺激因子和其受体的表达率分别是62.96%、66.67%。其中,G-CSF和G-CSFR在同一样本中均阳性表达者16例(59.26%),均阴性表达者8例(29.63%)。G-CSF阳性和G-CSFR阴性表达1例(3.70%),G-CSF阴性和G-CSFR阳性表达2例(7.41%)。G-CSF和GCSFR的表达与性别、年龄、临床分期和病理分型均无相关性(P>0.05)。结论 G-CSF,G-CSFR在儿童肾母细胞瘤中高表达,无论性别、年龄、临床分期以及病理类型,均应检测肿瘤G-CSF、G-CSFR表达,对于G-CSFR阳性表达的患者,建议使用G-CSF时,应慎重考虑其对肿瘤的影响。

Objective To determine the expression of granulocyte colony-stimulating factor（G-CSF） and its receptor（G-CSFR） in children Wilm＇s tumor.Methods Tumor samples of 27 children with identified Wilm＇s tumor who hospitalized in our department from January 2009 to June 2011 were collected.Immunohistochemistry was used to detect the expression and distribution of G-CSF and its receptor in these tumor specimens.Results G-CSF and its receptor were expressed in 62.96% and 66.67% respectively in Wilm＇s tumor specimens.Co-expression of G-CSF and G-CSFR in a same sample were found in 16 cases（59.26%）.There were 8 cases（29.63%） showing no expression of G-CSF and G-CSFR.One case（3.70%） had expression G-CSF but not its receptor,while 2 cases（7.41%） had expression of the receptor but no G-CSF.G-CSF and G-CSFR expression had no significant correlation with gender,age,clinical stage and pathology types（P0.05）.Conclusion G-CSF and G-CSFR are highly expressed in children Wilm＇s tumor regardless of gender,age,clinical stage and pathology types of tumor.Their expression should be detected as a routine examination.For the G-CSFR-positive patients,application of G-CSF should be carefully considered for its impact on the tumor.