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地塞米松诱导的危重病性肌病的细胞死亡方式 被引量：5

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摘要 目的探讨地塞米松诱导的危重病性肌病的细胞死亡方式。方法将SD大鼠分为健康对照组和实验组;实验组又分为7、9、11 d 3个时相点(n=10)。实验组采用5 mg/kg地塞米松连续腹腔注射,每天1次,健康对照组腹腔注射等量的生理盐水。采用肌功能缺损评分判定肌功能缺损情况。利用免疫组化和Western blot检测比目鱼肌activecaspase-3、PARP、pULK的表达情况。结果①健康对照组无肌肉功能缺损症状;而实验组7 d时大鼠70%出现肌肉功能缺损症状,9、11 d时全部出现明显的肌肉功能缺损症状,以11 d时相点缺损程度最为严重。②各组active caspase-3、PARP均为阴性表达;健康对照组可见少量pULK阳性表达细胞,实验组pULK表达显著增强,以11 d时相点表达增强最为明显。结论地塞米松诱导的危重病性肌病中无active caspase-3、PARP表达,以pULK表达为主,提示该病可能以自噬性死亡为主要死亡方式。
作者 袁静 梁佳 赵颂 包翠芬
机构地区 辽宁医学院附属第一医院神经内科 辽宁医学院科学实验中心
出处 《第三军医大学学报》 CAS CSCD 北大核心 2012年第5期453-455,共3页 Journal of Third Military Medical University
基金 辽宁省教育厅计划项目(2009A462)
关键词 地塞米松 肌疾病 半胱氨酸蛋白酶-3 多腺苷二磷酸核糖聚合酶 磷酸化自噬相关蛋白1抗体 大鼠
分类号 R746.102 [医药卫生—神经病学与精神病学] R329.28 [医药卫生—人体解剖和组织胚胎学]
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参考文献12

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共引文献1

同被引文献55

  • 1李艳,黄兆民.制动对骨骼肌的影响及机制[J].中国康复理论与实践,2006,12(12):1024-1025. 被引量：16
  • 2赵微,陈良安.机械通气患者营养支持[J].中国临床营养杂志,2007,15(2):116-121. 被引量：11
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引证文献5

二级引证文献14

第三军医大学学报
2012年 第5期

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