肾小球微小病变与局灶节段性肾小球硬化症足细胞中线粒体形态的定量研究(英文)

Stereological analysis on mitochondria in podocytes of minimalchange disease and focal segmental glomerulosclerosis

目的探索肾小球微小病变(MCD)与局灶节段性肾小球硬化症(FSGS)两种肾小球病中足细胞内线粒体的形态学差异。方法选择5例MCD和5例FSGS病例,在透射电镜放大30000倍的情况下,根据等距离曲线移动原则对每个病例拍照40张。根据Merz曲线和Gunderson测试系统选择线粒体和足细胞,测算并获得包括线粒体截面面积(A)、周长(C)、体积密度(VV)及面数密度(NA)等指标进行分析。结果 MCD和FSGS足细胞中线粒体相关参数的变异度及P值如下:A:0.47vs1.73,P=0.970;C:4.89vs24.71,P=0.590;VV:0.49vs1.74,P=0.946;NA:0.26vs0.58,P=0.602。结论 FSGS和MCD足细胞中线粒体的A、C、VV和NA等参数无统计学差异(所有P>0.05),但FSGS中所有参数的变异度均比MCD中的更明显,仍需进一步进行体视学研究。

Objective To explore the difference of mitochondria in podocytes between minimal change disease（MCD） and focal segmental glomerulosclerosis（FSGS） by stereologic analysis. Methods We selected 5 cases of primary MCD and 5 cases of primary FSGS whose diagnosis were definite and had no any other complicated nephropathy. For each case, we took 40 photos under the magnification of 30 000 according to the rule of equidistant zigzag movement. Merz curve and Gunderson testing system were set to select mitochondria and podocytes and the associated parameters were obtained including profile area（A） , circumference （ C）, volume fraction（ Vv） , and numerical density per area（ NA ） of mitochondria. Results The standard deviation value and P value of the parameters were as following in MCD vs FSGS： A（0.47 vs 1.73, P =0.970）, C（4.89vs24.71, P =0.590）, Vv（0.49vs 1.74, P =0.946）, andNA（0.26 vs O. 58, P = 0. 602） Conclusion The parameters of A, C, Vv, and NA did not demonstrate statistically significance（ all P 〉 0.05 ） , but all of them showed larger value of standard deviation in FSGS cases by comparison with that of MCD.