高效液相色谱法测定马拉韦罗片的含量

Determination of maraviroc tablets by high performance liquid chromatography

目的建立高效液相色谱法测定马拉韦罗片的含量。方法色谱柱:Shimadzu C18柱(250 mm×4.6 mm,5μm),流动相:乙腈-0.01 mol.L-1磷酸二氢钾溶液(25∶75,V/V),柱温:(35.0±1.0)℃,流速:1.0 mL.min-1,检测波长:197 nm。结果马拉韦罗的线性范围为0.1～0.5 g.L-1(r=0.999 7),平均回收率为(99.83±0.60)%(n=9)。结论本方法简便、准确,可用于马拉韦罗片的含量测定。

AIM To establish a method for content determination of maraviroc tablets by high performance liquid chromatography.METHODS A Shimadzu C18 column（250 mm × 4.6 mm,5 μm）was used with the mobile phase of acetonitrile-0.01 mol·L-1 potassium dihydrogen orthophosphate solution（25：75,V/V）.Column temperature was（35.0 ± 1.0）℃.Flow rate was 1.0 mL·min-1.UV detection wave length was 197 nm.RESULTS The linear range of maraviroc tablets was 0.1-0.5 g·L-1（r = 0.999 7）,with average recovery was（99.83 ± 0.60）%（n = 9）.CONCLUSION The established method is simple,accurate and suitable for the quality control of maraviroc tablets.