Tribbles相关蛋白3及磷酸化丝氨酸/苏氨酸激酶在舌体鳞状细胞癌中的表达及临床意义

Expression and clinical significance of TRB3 and p-AKT in oral tongue squamous cell carcinoma

目的研究Tribbles相关蛋白3(tribblesrelated protein 3,TRB3)及磷酸化丝氨酸/苏氨酸激酶(phospho-AKT,p-AKT)在人舌体鳞状细胞癌(oraltongue squamous cell carcinoma OTSCC)中的表达及临床意义。方法采用RT-PCR及Western blot方法检测18例OTSCC及对应癌旁黏膜组织中TRB3及p-AKT的表达,免疫组化方法检测128例OTSCC及30例癌旁黏膜石蜡标本中TRB3及p-AKT的表达,分析其表达与OTSCC预后的关系。结果 RT-PCR显示TRB3 mRNA在15例OTSCC组织表达较癌旁黏膜组织中升高(83.3%,15/18);Westernblot显示TRB3蛋白及p-AKT蛋白在13例OTSCC组织中的表达均较癌旁黏膜组织中升高(72.2%,13/18):免疫组化显示128例OTSCC标本中,TRB3蛋白高表达率为49.2%(63/128),p-AKT蛋白高表达率为68.8%(88/128),均较癌旁黏膜表达增高(P均≤0.001),且均与OTSCC的T分级、N分级及复发相关,p-AKT蛋白及TRB3蛋白的表达呈负相关(r=-0.27,P<0.01)。结论 TRB3及p-AKT在OTSCC中高表达并且与肿瘤预后相关。

OBJECTIVE To investigate the expression and clinical significance of TRB3 and p-AKT in oral tongue squamous cell carcinoma（OTSCC）. METHODS Expression of TRB3 mRNA in 18 paired OTSCC tissue and adjacent noncancerous mucosa was assessed using reverse transcription polymerase chain reaction（RT-PCR）.Western blot and immunohistochemistry assays were applied to detect expression of TRB3 and p-AKT protein.RESULTS In 18 paired tissues,expression of TRB3 mRNA in 15 （83.3%） OTSCC tissues were significantly higher than that in adjacent noncancerous mucosa.And expression of TRB3 protein and p-AKT protein were both significantly higher in OTSCC tissue than in adjacent noncancerous mucosa in 13/18（72.2%） Western blot assays. Immunohistochemical assays showed high expression of TRB3 protein in 63/128（49.2%） and p-AKT protein in 88/128（68.8%） paraffin-embedded OTSCC samples. Expression of TRB3 and p-AKT were significantly higher in OTSCC tissues than that in adjacent noncancerous tissues（both P0.001）.The Expression of TRB3 and p-AKT were significantly correlated with tumor pathological T stage,N stage and tumor recurrence.The expression level of TRB3 protein showed a significant negative relation with the expression level of p-AKT （r=-0.27,P0.01）.CONCLUSION TRB3 and p-AKT were overexpressed in OTSCC and were related to tumor prognosis.Expression of p-AKT was correlated negatively with expression of TRB3.