男性不育患者精浆和血清中锌铅镉丙二醛含量及超氧化物歧化酶活力的研究

Zinc,lead,cadmium,malondialdehyde contents and superoxide dismutase activity in serum and seminal plasma in male infertily patients

目的研究男性不育患者精浆中超氧化物歧化酶(SOD)活力、丙二醛(MDA)及锌、铅、镉含量的变化与精浆抗氧化能力的关系,为男性不育症患者寻找病因及诊治方法提供依据。方法选择精液常规检查异常的男性不育者120例,为不育组;精液常规检查正常者100例,为对照组。SOD活力采用黄嘌呤氧化酶比色法测定,MDA采用硫代巴比妥酸比色法测定,锌、铅、镉采用原子吸收分光光度计法进行测定。结果男性不育症患者精浆中锌、铅、镉、MDA含量和SOD活力分别为(27.18±7.88)μg/L,(1.98土0.49)ng/L,(9.03±3.36)ng/L,(150±11.9)nmol/L,(210±41)U/L。对照组精浆中锌、铅、镉、MDA含量和SOD活力分别为(46.50±6.77)μg/L,(0.85±0.33)ng/L,(7.59±2.11)ng/L,(98±12)nmol/L,(361±103)U/L,其中铅和MDA含量显著高于对照组,锌含量和SOD活力显著低于对照组(P<0.01);镉含量较对照组高,但差异无统计学意义。男性不育症患者血清中锌含量低于对照组,MDA含量高于对照组(P<0.01);铅、镉含量和SOD活力与对照组比较无显著性差异。结论男性不育者精浆中铅含量增高可导致精子细胞过氧化反应增强,抗氧化物锌和SOD消耗增加,脂质过氧化代谢产物MDA增多,对精子造成损伤,使精子质量降低。

Objective： To study the changes of zinc, lead, cadmium and malondialdehyde （MDA） contents and su- peroxide dismutase （SOD） activity in serum and seminal plasma in male infertility patients,and to provide the information for diagnosis and treatment of male infertility patients. Methods： A total of 120 male infertility patients with abnormal semen analysis were recruited as infer- tility group and 100 normal men with normal semen analysis as control group. The xanthine oxidase colori- metric analysis was used to determine SOD activity. The MDA content was assayed by the thiobarbituric acid colorimetry. The zinc, lead and cadmium contents were determined by using atomic absorption spectrometric method. Results： In the seminal plasma, the zinc content [（27. 18 ± 7. 88） μg/L] （x± s） and SOD activity [（210±41） U/L）] in infertility group were significantly lower than those in the control group [（46.50± 6.77） μg/L and （361_--＋103）U/L respectively）] （P^0.01）. The lead [（1.98-＋-0.49） ng/L] and MDA [- （150±11.9） nmol/L] contens in infertility group were significantly higher than those in the control group [（0.85±0.33） ng/L and （98 ± 12）nmol/L respectively）] （P〈0.01）. The cadmium content [（9.03± 3.36） ng/L] was higher in infertility group than that in the control group [（7.59±2.11） ng/L], but there was no statistical significance （P〉0.05）. In the serum, zinc content [（0.59±0.31） ng/LJ was signilicantly lower than that in the control group [（0.93±0. 60） μg/L] （P〈0.01）. The MDA content [（160±71） nmol/L] was significantly higher than that in the control group [（110±12） nmol/L] （P〈0.01）. The lead [（6.73±4.62） ng/L] and cadmium [（0.86±0.35） ng/L] content and SOD activity [（470±51） U/ L] were not significant different with those in the control group [（4.43±2.23） ng/L, （0.69±0.28） ng/L and （480±76） U/L respectively] （P〉0.05）. Conclusions. The seminal plasma lead content increased in male infertility patients could lead to the sperm cells peroxidation enhancement. The increased consumption of antioxidants i. e. zinc and SOD and enhancement of the lipid peroxidation metabolites i. e. MDA would damage sperms and lower sperm quality.