期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

食管癌肿瘤条件培养新生血管形成机制的探讨 被引量：2

Angiogenesis mechanism of esophageal squamous cell cancer in different culture condition esophageal squamous cell carcinoma
原文传递
导出
摘要 目的:探讨Survivin、Survivin-ΔEx3及VEGF在食管癌细胞诱导血管形成中作用的相关性。方法:用食管鳞癌肿瘤条件培养基培养人脐静脉内皮细胞,用ELISA法检测肿瘤条件培养基中VEGF蛋白的表达情况。用RT-PCR法检测内皮细胞中Survivin、Survivin-ΔEx3及VEGF的表达情况。结果:肿瘤条件培养基中VEGF蛋白表达随培养时间呈上升趋势。实验组人脐静脉内皮细胞中Survivin在更换肿瘤条件培养基后4及10h时表达明显增加,与对照组比较差异有统计学意义(CMvs RPMI:4h:1.019 7±0.058 8 vs 0.658 8±0.043 7,P=0.000 5;10 h:0.797 1±0.074 4 vs0.444 9±0.041 2,P=0.001 0),而Survivin-ΔEx3在实验组和对照组间的表达差异无统计学意义(CMvs RP-MI:4h:0.423 7±0.087 0 vs 0.294 0±0.043 1,P=0.109 1;10h:0.372 7±0.084 8 vs 0.226 4±0.009 2,P=0.078 1),VEGF在4及10h时表达明显下降,与对照组比较差异有统计学意义(CM vs RPMI:4h:0.276 7±0.065 8 vs 0.977 8±0.044 5,P=0.0000;10h:0.173 9±0.024 1 vs 0.917 8±0.124 7,P=0.004 8)。结论:食管癌肿瘤条件培养基可通过VEGF作用于人脐静脉内皮细胞表面的受体,进而增加人脐静脉内皮细胞中Survivin的表达,促进肿瘤新生血管形成,同时抑制血管内皮细胞中VEGF的表达,这为食管癌肿瘤血管形成的发生机制及防治提供一定的实验依据。 OBJECTIVE：To investigate the roles of Survivin,Survivin-ΔEx3 and VEGF in angiogenesis induced by esophageal squamous cell carcinoma（ESCC）.METHODS：VEGF expression in conditioned medium（CM） was examined by ELISA.Expression levels of the Survivin and Survivin-ΔEx3 in Human umbilical vein endothelial cells（HUVEC） which were cultured with CM from ESCC cell line（TE-5） were examined by RT-PCR.RESULTS：VEGF protein concentration in ESCC-derived CM was increased in time-dependent manner by ELISA.The expression level of Survivin in HUVEC increased significantly at 4 and 10 h（CM vs RPMI：4 h：1.019 7±0.058 8 vs 0.658 8±0.043 7,P=0.000 5;10 h：0.797 1±0.074 4 vs 0.444 9±0.041 2,P=0.001）.But Survivin-ΔEx3 had no significant change in each group（CM vs RPMI：4 h：0.423 7±0.087 0 vs 0.294 0±0.043 1,P=0.109 1;10 h：0.372 7±0.084 8 vs 0.226 4±0.009 2,P=0.078 1）.The expression level of VEGF decreased significantly at 4 h and 10 h,which has a very significant difference compared with the control group（CM vs RPMI：4 h：0.276 7±0.065 8 vs 0.977 8±0.044 5,P=0.000 0;10 h：0.173 9±0.024 1 vs 0.917 8±0.124 7,P=0.004 8）.CONCLUSION：The wild type Survivin but not Survivin-ΔEx3 is related to angiogenesis in ESCC.
作者 赵晶 刘丹 刘明娜 裴凤华 迟宝荣
机构地区 哈尔滨医科大学附属第二医院消化内科 吉林医学第一医院消化内科
出处 《中华肿瘤防治杂志》 CAS 2011年第23期1835-1838,共4页 Chinese Journal of Cancer Prevention and Treatment
基金 黑龙江省卫生厅资助项目(2009-115)
关键词 癌 鳞状细胞 酶联免疫吸附测定 内皮生长因子 SURVIVIN Survivin-ΔEx3 carcinoma squamous cell enzyme-linked immunosorbent assay endothlial growth factors Survivin Survivin-ΔEx3
分类号 R735.1 [医药卫生—肿瘤]
  • 相关文献

参考文献8

  • 1邹磊,李道堂,穆殿斌,李辉,罗景玉,刘曙光.食管鳞状细胞癌组织中Survivin和Cox-2及PCNA表达临床意义的研究[J].中华肿瘤防治杂志,2007,14(7):534-537. 被引量：3
  • 2Fangusaro JR, Jiang Y, Holloway MP, et al. Survivin, Sur vivin-2B, and Survivin-deItaEx3 expression in medulloblastoma biologic markers of turnout morphology and clinical outcome[J]. Br J Cancer,2005, 92(2) :359 365.
  • 3Tu SP, Jiang XH, Lin MC, et al. Suppression of survivin ex- pression inhibits in vivo tumorigenicity and angiogenesis in gas- tric cancer[J]. Cancer Res,2003, 63(22):7724-7732.
  • 4Folkman J. What is the evidence that tumors are angiogenesis dependent? [J]. J Natl Cancer [nst,1990, 82(1)14 6.
  • 5Nor JE, Christensen J, Mooney DJ, et al. Vascular endothelial growth factor (VEGF)-mediated angiogenesis is associated with enhanced endothelial cell survival and induction of Bcl-2 expres- sion[J]. AmJ Pathol,1999, 154(2) :375-384.
  • 6Sah NK, Khan Z, Khan GJ, et al. Structural, functional and therapeutic biology of survivin [J]. Cancer Lett,2006, 244(2):164-171.
  • 7Caldas H, Fangusaro JR, Boue DR, et al. Dissecting the role of endothelial Survivin-AEx3 in angiogenesis[J].Blood,2007, 109 (4) : 1479-1489.
  • 8Mesri M, Morales-Ruiz M, Ackermann EJ,et al. Suppression of vascular endothelial growth factor-mediated endothelial cell pro- tection by survivintargeting[J].Am J Pathol, 2001, 158(5): 1757-1765.

二级参考文献15

  • 1刘艳梅,黄建华,冯德云,郭新程.口腔鳞癌组织中Survivin蛋白的表达及与血管生成的关系[J].癌症,2005,24(11):1354-1357. 被引量：6
  • 2侯锐,石文君,曹薇.食管癌组织中Survivin和Bcl-2的表达与血管新生的关系[J].中华肿瘤防治杂志,2006,13(18):1403-1405. 被引量：1
  • 3Ambrosini G,Adida C,Altieri D C.A novel anti-apoptosis gene,survivin,expressed in cancer and lymphoma[J].Nat Med,1997,3(8):917-921.
  • 4Sobin L H,Wittekind C H.UICC TNM Classification of Malignant Tumors (6th edition)[M].New York:John Wiley,2002.
  • 5Altieri D C,Marchisio C.Survivin apoptosis:an interloper between cell death and cell proliferation in cancer[J].Lab Invest,1999,79(11),1327-1333.
  • 6Li F,Ambrosini G,Chu E Y,et al.Control of apoptosis and mitotic spindle checkpoint by survivin[J].Nature,1998,396(6711):580-584.
  • 7Kato J,Kuwabara Y,Mitani M.Expression of survivin in esophageal cancer:correlation with the prognosis and response to chemotherapy[J].Int J Cancer,2001,95(2):92-95.
  • 8Grabowski P,Kuhnel T,Muhr-Wilkenshoff F,et al.Prognostic value of nuclear survivin expression in oesophageal squamous cell carcinoma[J].Br J Cancer,2003,88(1):115-119.
  • 9Mega S,Miyamoto M,Li L,et al.Immunohistochemical analysis of nuclear survivin expression in esophageaL squamous cell carcinoma[J].Dis Esophagus,2006,19(5):355-359.
  • 10Buskens C J,Van Rees B P,Sivula A,et al.Prognostic significance of elevated cyclooxygenase-2 expression in patients with adenocarcinoma of the esophagus[J].Gastroenterology,2002,122(7):1800-1807.

共引文献2

同被引文献27

  • 1Biasco G,Derenzini E,Grazi G,et al.Treatment of hepatic metastases from colorectal cancer:many doubts,some certainties[J].Cancer Treat Rev,2006,32(3):214 228.
  • 2Matsumoto S,Tanaka F,Sato K,et al.Monitoring with a non-invasive bioluminescent in vivo imaging system of pleural metastasis of lung carcinoma[J].Lung Cancer,2009,66(1):75 79.
  • 3Morimoto-Tomita M,Ohashi Y,Matsubara A,et al.Mouse colon carcinoma cells established for high incidence of experimental hepatic metastasis exhibit accelerated and anchorage-independent growth[J].Clin Exp Metastasis,2005,22(6):513 521.
  • 4Ntziachristos V,Ripoll J,Wang L V,et al.Looking and listening to light:the evolution of whole-body photonic imaging[J].Nat Biotechnol,2005,23(3):313 320.
  • 5Baban C K, Cronin M, Akin A R, et al. Bioluminescent bacterial imaging in vivo [ J]. J Vis Exp, 2012 (69): e4318.
  • 6He Y,Wang R K,Xing D.Enhanced sensitivity and spatial resolution for in vivo imaging with low-level light-emitting probes by use of biocompatible chemical agents[J].Opt Lett,2003,28(21):2076 2078.
  • 7Rice B W,Cable M D,Nelson M B.In vivo imaging of light-emitting probes[J].J Biomed Opt,2001,6(4):432440.
  • 8Chen C H,Yeh Y C,Wu G J,et al.Tracking the rejection and survival of mouse ovarian iso-and allografts in vivo with bioluminescent imaging[J].Reproduction,2010,140(1):105 112.
  • 9Giubellino A,Woldemichael G M,Sourbier C,et al.Characterization of two mouse models of metastatic pheochromocytoma using bioluminescence imaging[J].Cancer Lett,2012,316(1):46 52.
  • 10Minn A J,Gupta G P,Siegel P M,et al.Genes that mediate breast cancer metastasis to lung[J].Nature,2005,436(7050):518 524.

引证文献2

二级引证文献4

中华肿瘤防治杂志
2011年 第23期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部