一个新的水稻叶绿素缺失黄叶突变体的特征及基因分子定位

Characteristics and molecular mapping of a novel chlorophyll-deficient yellow-leaf mutant in rice

从粳稻"嘉花1号"60Coγ射线辐照的后代中筛选到一个叶绿素缺失黄叶突变体(yl11),与野生型"嘉花1号"相比该突变体表现为全生育期植株叶片呈黄色,叶绿素含量以及净光合速率明显下降,叶绿体发育不完善,并且伴随着株高等主要农艺性状的变化。遗传分析表明,该突变性状受一对隐性核基因(yl11)控制。该突变体与籼稻"培矮64S"杂交生产的F2、F3群体中的分离出突变体型920个单株作为定位群体,利用SSR和InDel分子标记将yl11基因定位在水稻第11染色体长臂上的MM2199和ID21039分子标记之间,其物理距离约为110kb,目前该区域内没有发现与水稻叶绿素合成/叶绿体发育相关已知功能基因。研究结果为今后对该基因的克隆和功能分析奠定了基础。

A yellow-leaf mutant（yl11） with chlorophyll-deficient in rice（Oryza sativa L.） was selected from the progeny of a japonica rice variety ＂Jiahua 1＂ treated with 60Co γ-radiation.In comparison with the wild-type parent,＂Jiahua 1＂,the mutant had yellow-leaves at whole growth stages and displayed significantly decreased in chlorophyll content and net pho-tosynthetic rate.Underdeveloped chloroplast and alterations of the major agronomic traits,such as plant-heights,were also observed in the mutant.Genetic analysis confirmed that the yellow-leaf mutant trait was controlled by a single recessive nuclear gene（yl11）.Using SSR and In/Del molecular markers and 920 F2 and F3 plants from the cross of yl11 with the in-dica variety Peiai 64S,the yl11 was mapped between the molecular markers MM2199 and InDel21039 with a physical dis-tance of 110 kb on the long arm of chromosome 11,in which no known functional genes for chlorophyll synthesis or chloroplast development in rice has been found.These findings will provide a foundation for the cloning and functional analysis of this gene in the future.