阿托伐他汀促进大鼠体内Ⅰ型胶原蛋白支架血管化

Promoting effect of atorvastatin on vascularization for type I collagen scaffold in rat

探讨阿托伐他汀能否作为促血管新生药物促进组织工程移植物的血管化。方法：通过伊红染色、扫描电镜观察I型胶原蛋白支架的形态及表面结构，然后将支架植入大鼠体内，阿托伐他汀灌胃。做免疫组织化学显色和RT-PCR，检测血管内皮生长因子（VEGF）和CD34蛋白和基因的表达情况。结果：伊红染色可见，支架结构疏松、多孔。扫描电镜可见支架表面光滑，有少许皱褶。免疫组织化学结果可见CD34阳性细胞多位于支架边缘，均匀分散于胞膜中，VEGF染色阳性颗粒分布于细胞质或细胞外基质中。实验组阳性细胞表达较对照组多。RT-PCR结果显示实验组VEGF的基因表达较对照组高，差异具有统计学意义。结论：阿托伐他汀可以促进大鼠体内Ⅰ型胶原蛋白支架中VEGF基因的表达，刺激VEGF的分泌，增加CD34阳性细胞数量。

： To explore whether atorvastatin can promote the neovascularization. Methods： The morphology and surface structure of type I collagen scaffold were observed by eosin staining and scanning electron microscopy, and then subcutaneously transplanted into rats. Subsequently, the animals were intragastric administrated with atorvastatin. 14 days later, the expressions of VEGF and CD34 were detected using immunohistochemistry. Results： The scaffold was loose and porous, with a smooth surface and a little reductus. CD34 positive staining cells were found in the edge of scaffold, and scat- tered evenly in cell membrane, and VEGF positive staining cells distribueted in cytoplasm or cell membrane. The sum of positive staining cells in the experimental group was bigger than that in the control group. The RT-PCR result informed that the expression level of VEGF gene in the experimental group was higher than that in the control group, with a significant difference. Conclusion： Atorvastatin can promote the expression of VEGF gene in type I collagen protein scaffold, facilitate the secretion of VEGF, and increase CD34 positive staining cells.