成年鼠和幼鼠卵巢组织冻融后的形态学变化

Morphological changes of ovarian tissues from adult and young mice after vitrification

选择合适发育阶段卵巢组织进行冷冻，以提高冷冻保存效果。方法：分别对10日龄幼鼠和6周龄成年鼠的卵巢组织进行玻璃化冷冻保存，比较卵巢组织中各级卵泡在冻融前后形态学的改变和存活率的变化。结果：幼鼠卵巢组织冻融后各级形态正常卵泡和活卵泡所占的比例与新鲜卵巢组织没有差异，而成年鼠卵巢组织冻融后各级形态正常卵泡和活卵泡所占的比例低于新鲜卵巢组织。卵巢组织冻融后肾被膜下移植实验显示，幼鼠卵巢组织建立血供的成功率高于成年鼠的卵巢组织。结论：幼鼠卵巢组织的玻璃化冷冻效果好于成年鼠，建议在青春期前进行卵巢组织的冷冻保存。

To improve the cryopreservation effect of freezing on ovarian tissues at appropriate developmental sta- ges. Methods： The ovarian tissues from 10-day-old and 6-week-old mice were frozen by vitrification, and then were com- pared by introducing survival rate and morphological changes of the follicles. Results： The percentages of morphologically normal follicles and live follicles in thawed ovarian tissues from young mice had no significant difference with those in the fresh ovarian tissues from young mice, while vitrification significantly reduced the percentages of morphologically normal fol- licles and live follicles in the thawed ovarian tissues from adult mice. After the thawed ovarian tissues were transplanted into the kidney capsule, the rate of the blood supply establishing in the ovarian tissues from young mice was significantly higher than that from adult mice. Conclusion： The vitrification effect of ovarian tissues from young mice is obviously better than that from adult mice, suggesting ovarian tissues should be cryopreserved before puberty.