生长休止蛋白7在大鼠内嗅皮质发育过程中的表达

Expression of growth arrest-specific protein 7 in the entorhinal cortex of rat during development

研究生长休止蛋白7（Gas7）在大鼠内嗅皮质（EC）不同发育阶段的表达。方法：采用免疫组织化学方法观察Gas7在SD大鼠胚胎第18．5天（E18．5d）、新生第0天（P0d）、P7、P14、P21和成年EC中的表达。结果：Gas7免疫阳性产物出现在E18．5d大鼠ECⅡ板层的表面，至P7d向Ⅱ板层内分布，并可见清晰的Gas7阳性细胞，Gas7免疫阳性产物表达于胞膜和胞质。到P14d，Gas7阳性细胞深染并向内扩散至Ⅵ板层，其中Ⅱ板层阳性细胞最密集，形态上以锥体样细胞和星形细胞多见。P21d大鼠内嗅皮质各板层Gas7免疫阳性反应均弱于P14d，至成年，Gas7免疫阳性细胞基本上局限于Ⅱ板层内，呈中等度着色。Gas7在围产期大鼠EC的表达呈现先增高后降低的趋势，最高点在P14d，至P21d其表达强度和分布接近成年水平。结论：Gas？在大鼠内嗅皮质发育过程中的表达呈现时空特异性，提示Gas7可能参与了内嗅皮质发育过程中结构形成和功能成熟的调控。

To study the expression of growth arrest-specific protein 7 （Gas7） in the entorhinal cortex （EC） of rat during developmental. Methods： Immunohistochemistry was used to determine the expression of Gas7 in the rat EC from six developmental stages： embryonic day 18. 5 （E18. 5）, postnatal day 0 （P0）, P7, P14, P21 and aduh. Results： Gas7 immu- nostaining was first detected on the surface of layer Ⅱ in EC, and then extended inward into the layer Ⅱ by P7 when clear Gas7 immunostaining ceils were seen. Gas7 expression was located in cytoplasm and cytolemma. By P14 d, Gas7 positive cells were heavily stained and distributed in layer II-VI with more compacted cells in layer Ⅱ. Morphologically, pyramidal- like and stellate cells were more numerous. Compared to P14 d, Gas7 immunostaining was obviously declined in the EC of P21 d rats. Moderate Gas7 immunostaining was mainly limited in layer Ⅱ in adult rat EC, Gas7 expression was first progres- sively adscendent to the highest level in P14 d EC, and then declined to moderate level in adult rat EC. Conclusion： Gas7 expression in EC showed both position and time dependency, indicating that Gas7 is likely to be involved in morphogenesis and functional matu- ration during EC; development.