摘要
观察短暂性缺氧后鼠脑神经源性分化因子(NeuroD)表达量的变化,探讨其在神经系统再生中的可能作用。方法:通过延迟剖宫产术建立胎鼠宫内窘迫模型,原位杂交技术检测不同时间海马结构NeuroD mRNA表达量的变化。结果:原位杂交结果显示,模型组无论在齿状回颗粒下层(SGZ)还是CAl区,NeuroD在P13、P20、P27d的表达量与对照组相比均增高。结论:短暂性缺氧后诱导NeuroD mRNA增高,可能参与了神经系统的再生。
To observe the influence of perinatal transient hypoxia on the mRNA expression of neurogenic differen- tiation (NeuroD) in rats. Methads: Transient hypoxia was prepared by asphyxia of pregnant Sprague-Dawley rats for 10 min and delayed cesarean, and neonatal rats survived for postnatal 13, 20, 27 days, respectively. The mRNA expression of Neu- roD was detected in the subgranular zone of dentate gyrus (SGZ) and CA1 layer of the hippocampus by in situ hybridization. Results: Hypoxia significantly enhanced mRNA expressions of NeuroD in SGZ and CA1 of the hippocampus at P13, 20 and 27. Conc/us/on: The expression of NeuroD increases after exposure to hypoxia, which rmy be involved in nerve regeneration,
出处
《解剖学杂志》
CAS
CSCD
北大核心
2012年第1期79-81,共3页
Chinese Journal of Anatomy
基金
福建省青年人才基金(2008F3048)
福建省教育厅项目(JA08108)
福建医科大学教授学术发展基金