摘要
采用批量培养的方法研究洋河水库底泥浸出液对洋河水库微囊藻和鱼腥藻生长的影响.结果表明,厌氧条件底泥浸出液对鱼腥藻生长速率有促进作用,添加2%、20%、50%的厌氧底泥浸出液的鱼腥藻生长速率比M11培养基中分别高出36.6%、47.2%、36.0%;而添加厌氧底泥浸出液对微囊藻生长有抑制作用,微囊藻最大生物量随底泥浸出液的添加量增加而减小.与添加厌氧底泥浸出液相比,添加好氧条件的底泥浸出液对鱼腥藻生长速率也有一定的促进作用,添加20%的好氧底泥浸出液的鱼腥藻生长速率比M11培养基中高出37.2%;而添加好氧底泥浸出液对微囊藻生长影响不明显.在添加厌氧底泥浸出液的培养基中增加铁,则微囊藻的最大生物量显著增加,表明底泥浸出液对微囊藻生长的影响可能是较高浓度的有机质与铁的结合影响了微囊藻对铁的利用.
Batch culture experiments were used to study the effect of leachate from sediment of Yanghe reservoir on the growth of Microcystis and Anabaena isolated from Yanghe reservoir.The results showed that the growth of Microcystis was significantly inhibited when the addition of anaerobic leachate from sediment in M11 culture medium was high(≥20% V/V).The maximum biomass of Microcystis was lower than that grown in pure M11 culture medium.But there was an obvious promotion on the growth of Anabaena when anaerobic leachate from sediment was added.The growth rate of Anabaena increased 36.6%,47.2% and 36.0%,respectively,compared to M11 culture medium when adding 2%,20% and 50%(V/V) anaerobic leachate.Compared to adding anaerobic leachate,aerobic leachate had no influence on the growth of Microcystis,while the growth rate of Anabaena was promoted by aerobic leachate.The growth rate of Anabaena increased 37.2% compared with M11 when adding 20%(V/V) of aerobic leachate.But after addition of Fe-citrate to the mixed culture medium(50% M11 culture medium +50% anaerobic leachate),the maximum biomass of Microcystis significantly increased.The results suggest that high organic matter concentration decreases iron availability for Microcystis.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2012年第3期844-848,共5页
Environmental Science
基金
环境保护公益项目(200809145)
国家自然科学基金项目(50938007
51078341)
关键词
洋河水库
微囊藻
鱼腥藻
底泥
铁
Yanghe reservior
Microcystis
Anabaena
sediment
iron