3种杀真菌剂对AM真菌侵染和黄芩生长的影响

Effects of Three Fungicides on Arbuscular Mycorrhizal Fungal Infection and Growth of Scutellaria baicalensis Georgi

为了合理施用杀真菌剂,充分利用AM(arbuscular mycorrhiza)真菌资源提高中药材产量和品质,减少农药投入和环境污染.本试验在土培条件下,以非灭菌土为生长基质,研究了喷施苯菌灵、苯醚甲环唑和氟硅唑对AM真菌(摩西球囊霉Glomusmossea)侵染和黄芩(Scutellaria baicalensis)生长的影响.结果表明,同一施药条件下,接种摩西球囊霉总体上表现出促进黄芩生长的趋势,但菌根效应因杀真菌剂不同而有差异.不同施药条件下,未接种黄芩生长受到抑制,接种株中,喷施苯菌灵,植株地上部K和地下部Fe含量显著降低;喷施苯醚甲环唑,植株全N、地上部K、地下部黄芩苷和Ca含量显著降低;喷施氟硅唑,菌根侵染率、植株全P、黄芩苷、K、地上部Cu、地下部全N、Ca、Zn和Fe含量显著降低.氟硅唑对摩西球囊霉和黄芩生长抑制效果大于苯醚甲环唑和苯菌灵.说明氟硅唑对摩西球囊霉毒性大.因此,为了减轻杀真菌剂对AM真菌的危害,发挥菌根效应,在达到防治病害的基础上应选用对AM真菌毒性小的杀真菌剂.

To make full use of arbuscular mycorrhizal（AM） fungal resources to increase the quantity and quality of Chinese medicinal materials and reduce the use of fungicide and environmental pollution,this research was carried out under pot culture condition to study the effects of benomyl,difenconazole and flusilazol on AM fungal（Glomus mosseae） colonization and the growth of Scutellaria baicalensis.The results showed that Glomus mosseae generally promoted the growth of host plant under the same fungicide application,and the effect of Glomus mosseae was different with different fungicides.The growth of non-mycorrhizal Scutellaria baicalensis was inhibited by applying with different fungicides.On mycorrhizal plants,contents of K in shoot and Fe in root significantly decreased under benomyl treatment;the contents of total N,K in shoot,Baicalin and Ca in root significantly decreased under difenconazole treatment;mycorrhizal colonization,the contents of total P,Baicalin,K and Cu in shoot,total N,Ca,Zn and Fe in root significantly decreased under flusilazol.The inhibitory effects of flusilazol on the Glomus mosseae colonization and growth of Scutellaria baicalensis were greater than those of difenconazole and benomyl.It is reasonable to choose less toxic fungicides in Scutellaria baicalensis cultivation to reduce the harmful effects on arbuscular mycorrhizal fungi.