ω-3多不饱和脂肪酸对颅脑损伤伴休克大鼠全身炎性反应及肠黏膜屏障的影响

Effects of w-3 polyunsaturated fatty acids on systemic inflammatory response and gut barrier in rats with traumatic brain injury and hemorrhagic shock

目的观察w-3多不饱%脂肪酸（tO-3PUFA）对颅脑损伤伴失血性休克（TBIS）大鼠全身炎性反应及肠黏膜屏障的影响。方法将36只雄性Wistar大鼠按随机数表法随机分成假手术组、TBIS模型组%tO．3PUFA治疗组，每组12只。于建立颅脑外伤合并休克模型术前12h及2h经尾静脉给to-3PUFA治疗组大鼠注射2ml／kgw-3PUFA，同时给假手术组%TBIS模型组大鼠注射生理盐水。在模型建立后120min采集标本，采用酶联免疫吸附法检测血清肿瘤坏死因子（TNF）-a、8-异前列腺素F2a（8-iso—PGF，a）、白细胞介素（IL）-1β及IL-10水平；HE染色观察小肠组织形态，评估小肠黏膜上皮损伤指数；检测肠系膜淋巴结、肝、脾、肺、肾等器官中标记大肠杆菌的检出率。结果TBIS模型组%oo-3PUFA治疗组血清TNF-a[（328．11±20．09）%（244．37±21．82）ng／m1]、8-iso—PGF2a[（263．47±55．19）%（176．354-41．63）pg／m1]、IL-1B[（27．06±2．61）%（18．91±1．78）μg／L]及IL-10[（7．63±1．29）%（9．524-1．66）μ∥L]水平，小肠黏膜上皮损伤指数（4．184-0．39%3．31±0．40）及多脏器荧光标记大肠杆菌的检出率（56．67％%35．00％）均显著高于假手术组[（38．15±6．37）ng／ml，（84．91±17．22）pg／ml，（2．52±0．83）μ∥L，（2．86±0．82）μ∥L，0．36±0．14，8．33％；P均〈O．01]。与TBIs模型组比较，PUFA治疗组血清TNF-d、-iSO—PGF2at及IL—lL水平，小肠黏膜上皮损伤指数及多脏器荧光标记大肠杆菌的检出率均显著降低（P均〈O．05），血清IL-10水平显著升高（P〈0．01）。结论w-3PUFA预处理可有效抑制TBIS大鼠全身炎性反应，减轻肠黏膜损伤。

Objective To investigate the effects of to-3 polyunsaturated fatty acids （ to-3 PUFA） on sys- temic inflammatory response and intestinal mucosa barrier in rats with traumatic brain injury and hemorrhagic shock （TBIS）. Methods A total of 36 male Wistar rats were equally randomized into 3 groups： sham operation group, TBIS model group, and w-3 PUFA pretreatment group. The serum levels of tumor necrosis factor-or （TNF-ct）, 8- iso-prostaglandin F2 a （8-iso-PGF2 a）, interleukin （IL）-19, and IL-10 were measured by enzyme linked immunosor- bent assay （ELISA）. HE staining was performed for morphological assessment of the intestinal tissue and evaluation of the intestinal mucosa damage index （IMDI）. The marked bacilli of the mesenteric lymph nodes, lung, liver, spleen, and kidney tissue were counted under a fluorescent microscope. Results Compared with those in the sham group [ （38. 15 ＋6.37） ng/ml, （84. 91 ＋17.22） pg/ml, （2.52 _＋0. 83） ug/L, （2. 86 ＋0. 82） Ixg/L, 0. 36 ＋ 0. 14, and 8.33% ], the serum levels of TNF-a [ （328.11 ＋20. 09） and （244. 37 ＋21.82） ng/ml], 8-iso-PGF2a [ （263.47 ＋55.19） and （176.35 ＋41.63） pg/ml], IL-1[3 [ （27.06 ＋2.6l） and （18.91 ＋1.78） ug/L],IL-10 [ （7.63 ＋ 1.29） and （9.52 ＋ 1.66） ug/L], the IMDI （4.18 ＋0.39 and 3.31 ＋0.40）, and the positive rates of bacterial translocation （56.67% and 35.00% ） were significantly higher in both the TBIS model group and w-3 PUFA group （ all P 〈0. 01 ）. Compared with TBIS model group, the levels of TNF-a, 8-iso-PGF2a, and IL-1B, the IMDI, and the positive rate of bacterial translocation were significantly lower （ all P 〈 0.05 ） and the levels of IL-lO were significantly higher in the W-3 PUFA group （ P 〈 0.01 ）. Conclusion The supplementation of W-3 PUFA can remarkably inhibit the systemic inflammatory response and protect the integrity of intestinal mucosa in rat with TBIS,