ω-3多不饱和脂肪酸廿烷五烯酸单剂以及联合卡铂对人肺腺癌A-549细胞系增殖和凋亡的影响

Effect of eicosapentaenoic acid or eicosapentaenoic acid plus carboplatin on proliferation and apoptosis of human lung cancer cell line A-549

目的观察廿烷五烯酸（EPA）单剂以及与卡铂联合应用对人肺腺癌A-549细胞系增殖和凋亡的影响。方法分别用100μg／ml卡铂、80μg／mlEPA以及100μg／ml卡铂联合80μg／mlEPA孵育A-549细胞48h后，采用MTr法检测药物对h-549细胞增殖的抑制率，HE染色观察细胞形态学，流式细胞术定量分析细胞凋亡率。结果100μg／ml卡铂联合80μg／mlEPA对A-549细胞系的增殖抑制率为85．20％±5．00％，显著高于80μg／mlEPA（32．85％±3．00％，P=0．0001）或100μg／ml卡铂（53．25％±3．00％，P=0．0013）单独的作用。HE染色显示：药物干预后部分A-549细胞出现凋亡形态学改变。卡铂联合EPA组A-549细胞凋亡率为17．05％4-4．00％，显著高于单用卡铂组（9．49％±1．00％，P20．0252）。结论EPA可能具有增强卡铂抑制人肺腺癌A-549细胞系增殖、促进A-549细胞系凋亡的作用。

Objective To investigate the effects of eicosapentaenoic acid （EPA） or EPA plus carboplatin on the proliferation and apoptosis of human lung cancer cell line A-549. Methods A-549 cells were cultured by 100 μg/ml carboplatin, 80 μg/ml EPA, and their combination for 48 hours. MTT assay was used to determine the effect of EPA, carboplatin, or their combination on the proliferation of human lung cancer cell line A-549. The morphological changes of human lung cancer cell line A-549 were observed using HE staining, and apoptosis of A-549 cells was analyzed by flow cytometry. Results MTT assay showed that the proliferation inhibition rate of A-549 cells cultured with EPA plus carboplatin was 85.20% ± 5.00%, which was significantly higher than that of cells cultured with 80 ug/ml EPA （32. 85% ± 3.00%, P = 0. 0001 ） or 100 ug/ml carboplatin （53.25%±3.00%, P = 0.0013 ）. HE staining showed apoptosis in all three groups, whereas the apoptosis rate reached 17.05% ± 4.00% in the combination group, which was significantly higher than that in carboplatin group （9.49% ± 1.00% , P =0. 0252）. Conclusion EPA may enhance the effects of carboplatin in inhibiting the proliferation and promoting the apoptosis of human lung cancer cell line A-549.