摘要
通过构建东亚三角涡虫(Dujesia japonica)cDNA文库,随机挑选重组阳性克隆进行测序,对部分序列进行引物步移法测序,获得1个三角涡虫新基因——Rab蛋白基因(DjR),涡虫Rab蛋白cDNA全长2 141 bp,开放性阅读框(ORF)621bp,编码206个氨基酸,相对分子量为23.1 kD,等电点6.59,属亲水性蛋白,主要定位于细胞质中,在氨基酸第20和21位之间有信号肽剪切位点。有8个磷酸化位点。含有小G蛋白家族5个保守的鸟苷酸结合区域。同源性比较分析结果表明,其碱基序列与已经报道的其他23个物种的相似性为53%-90%,且符合种属之间的进化关系。
A cDNA library was constructed from Dujesia japonica adult stage.Clones were selected randomly from the cDNA library and were sequenced by using the method of expression sequence Tags（ESTs）.Novel genes were acquired by primer walking.The Rab gene sequence is 2 141 bp in length,contains an ORF of 621 base pairs and encodes a polypeptide of 206 residues with a predicted molecular weight about 23.1 kD and pI 6.59.Rab protein contain five guanine nucleotide binding domains（GNB,this domain plays a key role in molecular regulation,and are highly conserved among plants,yeasts and animals.The deduced amino acid sequence of Dujesia japonica Rab showed 53%-90% identity with the others species.
出处
《生物技术通报》
CAS
CSCD
北大核心
2012年第2期144-150,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(30670279
30970346)
