羊毛防毡缩用蛋白酶的化学修饰

Chemical Modification of Protease Used for Shrink Resistance of Wool

为减少防毡缩整理中蛋白酶对羊毛纤维主体结构的破坏作用,分别研究了戊二醛、微生物谷氨酰胺转氨酶(MTG)和水溶性碳二亚胺(EDC)对蛋白酶Savinase 16L的化学修饰,以期达到增大蛋白酶分子量,从而将水解作用限制在纤维表面的目的。主要通过体积排阻色谱、SDS-PAGE谱图以及荧光光谱研究修饰酶的分子量和结构变化。结果表明,戊二醛不能对蛋白酶分子进行有效修饰;MTG会被蛋白酶水解,无法催化酶分子间发生共价交联;而碳二亚胺既可以使蛋白酶分子间发生交联,又能将含有伯胺基的大分子修饰剂偶联到酶分子上。

Glutaraldehyde,microbial transglutaminase（MTG） and water-soluble carbodiimide（EDC） were employed to modify the protease used in anti-felting finishing of wool,with the purpose of increasing its molecular weight and restricting its action to the wool surface.The molecular weight distribution and structural changes of the modified protease were characterized by size exclusion chromatography,SDS-PAGE and fluorescence spectrum.The results showed that glutaraldehyde was not an efficient crosslinker for the protease,since no increase in the molecular weight was achieved.When MTG was used for the modification of the protease,it could be hydrolyzed by the protease and lost cross-linking function.EDC can be used to modify the protease.Moreover,polymers containing primary amine can be conjugated to the protease when EDC existed.