续断的HPLC指纹图谱研究

Study on HPLC Fingerprints of Dipsacus asper

目的:建立续断的高效液相色谱(HPLC)指纹图谱,并对不同产地续断的指纹特征进行比较,为其质量评价提供新的方法。方法:采用HPLC法测定了19批不同产地的续断样品,色谱柱为Ultimate XB-C1(8250mm×4.6mm,5μm),流动相为乙腈-0.05%磷酸水(梯度洗脱),柱温为25℃,流速为1mL·min-1,检测波长为212nm。并对不同产地样品的相似度进行了比较。结果:利用《中药色谱指纹图谱相似度评价系统(2004A版)》建立了续断药材的指纹图谱共有模式,标出了21个特征峰。除了10号样品,其他18批样品的相似度均较高。结论:本方法具有较好的重复性、精密度、稳定性,可为续断的质量控制提供科学依据。

OBJECTIVE： To establish HPLC fingerprints of Dipsacus aspen, and to compare the fingerprints of D. asper from different regions so as to set up new method for the quality control of D. aspen METHODS： 19 batches of samples from different regions were analyzed by HPLC. Ultimate XB-C18 （250 mm×4.6 mm, 5 μm） column was adopted with mobile phase consisted of acetonitrile-0.05%H2PO4 （gradient elution） at the flow rate of 1 mL· min^-1. The column temperature was 25 ℃, and the detective wavelength was set 212 nm. The similarity of samples from different regions was compared. RESULTS： The mutual mode of the fingerprints of 1）. asper was set up with ＂Similarity Evaluation System for Chromatographic Fingerprint of TCM＂ （2004 A version）, and 21 characteristic marks were marked. The samples had high similarity, except for No. 10 sample. CONCLUSION： The method has a good repeatability, precision and stability. It can provide scientific basis for the quality control of D. aspen