木芙蓉叶的质量标准研究

Study on Quality Standard of Hibiscus mutabilis

目的:建立木芙蓉叶的质量标准。方法:采用显微鉴别、薄层色谱(TLC)法对木芙蓉叶进行定性鉴别;采用高效液相色谱法对药材中有效成分芦丁进行含量测定。结果:木芙蓉叶显微特征明显;TLC斑点清晰、分离度好;芦丁进样量在0.0188～0.9412μg(r=0.9996)范围内与峰面积积分值呈良好线性关系,平均加样回收率为97.97%,RSD=1.97%(n=6)。结论:所建标准可用于木芙蓉叶的质量控制。

OBJECTIVE： To establish the method for the quality control of Hibiscus mutabilis. METHODS： Microscopic identification, TLC and HPLC were used to identify H. mutabilis quantitatively. HPLC method was adopted to determine the content of active component rutin. RESULTS： Microscopic characteristics of H. mutabilis was distinguishing. TLC spots were clear and well-separated. The linear range of rutin was 0.018 8-0.941 2 μg（r=0.999 6） with an average recovery rate of 97.97% （RSD= 1.97 %, n=6）. CONCLUSION： The etablished standard can be used to control the quality of H. mutabilis.