HPLC-LTQ Orbitrap MS对血液、尿液中精神药品的筛选及确证

Screening and Confirmation of Psychotropic Drugs in Blood and Urine by HPLC-LTQ Orbitrap MS

目的利用高效液相色谱-质谱联用(HPLC-LTQ Orbitrap MS)技术建立准确、快速筛选和确证血液、尿液中精神药品及其代谢物的方法。方法采用蛋白沉淀板处理样品,通过HPLC-LTQ Orbitrap MS对血液、尿液中的精神药品及其代谢物共56种化合物进行分离和检测,并考察其在血液和尿液中的最低检测限。建立精神药品精确相对分子质量数据库。结果所测定的56种化合物绝大部分最低检测限≤0.1 ng/mL。建立了包含精神药品及其代谢物共61种化合物的精确相对分子质量数据库。结论建立的方法准确、快速、灵敏度高,结合精确相对分子质量数据库,可应用于实际案例中精神药品的筛选和确证。

Objective To establish a screening and confirmation method for psychotropic drugs and their metabolites in human blood and urine by HPLC-LTQ Orbitrap MS.Methods The samples were pretreated with SiroccoTM protein precipitation plate,and then analyzed by HPLC-LTQ Orbitrap MS.The method was validated in terms of the limit of detection（LOD）.An accurate mass database was created for psychotropic drugs screening.Results The LOD for most of 56 determined compounds was ≤0.1 ng/mL.The accurate mass database included the accurate mass information of 61 psychotropic drugs.Conclusion The method is accurate,rapid,sensitive and the database is suitable for psychotropic drugs screening and confirmation.