补肾益髓中药复方对糖皮质激素性骨质疏松症大鼠肾组织Msx2 mRNA及蛋白表达的影响

Effect of Reinforcing Kidney to Replenish Marrow Traditional Chinese Herbs Compound on mRNA and Protein Expression of Msx2 in Kidney Tissues of Rats with Glucocorticoid-Induced Osteoporosis

目的:观察糖皮质激素性骨质疏松症(GIOP)大鼠肾组织Msx2 mRNA及蛋白表达,探讨GIOP的发病机制以及补肾益髓中药复方的疗效机理。方法:肌注地塞米松复制GIOP大鼠模型,实验设正常组、模型空白组、补肾益髓中药复方组、补中益气颗粒组、血府逐瘀胶囊组、骨疏康颗粒阳性对照组,造模及灌胃给药9周。应用XR-26型双能X线骨密度仪测定股骨骨密度,实时定量RT-PCR及Western Blot检测肾组织Msx2 mRNA及蛋白表达。结果:与正常组比较,模型空白组股骨骨密度明显降低(P<0.01),肾组织Msx2 mRNA及蛋白表达明显降低(P<0.01);与模型空白组比较,补肾益髓中药复方组股骨骨密度明显升高(P<0.01),肾组织Msx2 mRNA及蛋白表达明显上调(P<0.01)。结论:肾组织Msx2 mRNA及蛋白表达降低可能是GIOP的发病机制之一;补肾益髓中药复方可能通过上调肾组织Msx2 mRNA及蛋白表达,有效防治GIOP。

Objective：To observe mRNA and protein expression of Msx2 in kidney tissues of the rats with glucocorticoid-induced osteoporosis（GIOP）and discuss the pathogenesis of GIOP and the therapeutic effect and mechanism of reinforcing the kidney to replenish marrow traditional Chinese herbs compound.Methods：We established the GIOP rat models by intramuscular injection of dexamethasone.We divided all the rats into normal group,blank model group,reinforcing the kidney to replenish marrow traditional Chinese herbs compound group,reinforcing the middle to benefit qi granula group,removing blood-house stasis capsule group and Gushukang granula positive control group.After 9 weeks of establishing models and administrating by gavage,we detected femur bone mineral density by using the XR-26 dual energy X-ray absorptiometry.We detected mRNA expression of Msx2 in kidney tissues by Real-Time Quantitative RT-PCR and detected their protein expression by Western Blot.Results：Compared with normal group,femur bone mineral density of blank model group decreased significantly（P0.01）,mRNA and protein expression of Msx2 decreased significantly（P0.01）in kidney tissues;Compared with blank model group,femur bone mineral density of reinforcing the kidney to replenish marrow traditional Chinese herbs compound group increased significantly（P0.01）,mRNA and protein expression of Msx2 up-regulated significantly（P0.01）in kidney tissues.Conclusions：Maybe it is one aspect of the pathogenesis of GIOP that mRNA and protein expression of Msx2 decreases in kidney tissues;The reinforcing the kidney to replenish marrow traditional Chinese herbs compound can prevent and treat GIOP effectively by up-regulating mRNA and protein expression of Msx2 in kidney tissues.