蒙古黄芪凝集素诱导HL-60细胞凋亡作用研究

Effects of AMML Inducing Apoptosis in Human HL-60 Cells of Leukemia

目的:探讨蒙古黄芪凝集素(AMML)诱导HL-60细胞凋亡的作用和机制。方法:MTT法检测AMML对HL-60细胞的增殖抑制作用。显微镜下检测细胞凋亡形态的变化,应用流式细胞仪Annexin-V/PI双染法检测细胞凋亡,同时分析细胞周期。免疫细胞化学法检测Bcl-2、Caspase-3的表达。结果:MTT法显示AMML对HL-60细胞的生长具有明显的抑制作用。荧光显微镜下,细胞呈凋亡形态学变化,流式细胞仪检测结果为细胞凋亡率明显增高,出现G0/G1期阻滞。Bcl-2表达降低,Caspase-3表达增高。结论:AMML明显抑制HL-60细胞的生长,诱导细胞凋亡。可应用于抗肿瘤药物的开发。

Objective：To investigate the effect of AMML inducing apoptosis on human HL-60 cells of leukemia.Methods：The growing of HL-60 cells was measured by MTT colorimetric assay.The apoptosis rate and cell cycle were analyzed by flow cytometry.Bcl-2 and Caspase-3 expressions were stained by immunocytochemistry and determined by computerimage analysis system.Results：MTT showed that AMML could inhibit the growth of HL-60 cells.The results of flow cytometry indicated that the apoptosis rate of cells was significantly increased.Meanwhile,blockage of G0/G1 phase appeared.The expression of Bcl-2 was decreased and the expression of Caspase-3 was increased.Conclusions：AMML can inhibit the growth of HL-60 cells and induce the apoptosis of HL-60 cells.Thus,AMML may be used for the medicine of anti-cancer.