激光穴位照射对失眠大鼠下丘脑单胺类递质的影响

Effect of laser acupoint irradiation on monoamine neurotransmitter in the hypothalamus of insomnia rats

目的观察激光穴位照射对对氯苯丙氨酸(PCPA)所致失眠大鼠下丘脑单胺类神经递质5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、多巴胺(DA)、去甲肾上腺素(NE)含量的影响,探讨激光穴位照射治疗失眠的机制。方法将SD大鼠24只随机分为4组:对照组、模型组、安定组、激光组,每组6只。腹腔注射PCPA建立失眠大鼠模型,氦氖激光照射神门和三阴交穴各10 min,用荧光分光光度分析法检测下丘脑5-HT、5-HIAA、DA、NE的变化。结果激光组大鼠下丘脑5-HT、5-HIAA含量明显升高,与模型组比较差异有统计学意义(P<0.01),与安定组比较差异有统计学意义(P<0.05,P<0.01);NE、DA含量明显降低,与模型组比较差异有统计学意义(P<0.05,P<0.01),与安定组比较差异有统计学意义(P<0.05)。结论激光穴位照射可能是通过升高失眠大鼠下丘脑5-HT、5-HIAA,降低DA、NE来达到治疗失眠的目的。

Objective To observe the effect of laser acupoint irradiation on the content of monoamine neurotransmitter such as N-Acetyl-5-Hydroxytryptamine（5-HT）,5-Hydroxyindole-3-acetic acid（5-HIAA）,Dopamine（DA） and L-Noradrenaline（NA） in the hypothalamus of insomnia rats caused by DL-P-Chlorophenylalanine（PCPA）,and investigate the mechanism of laser acupoint irradiation treatment of insomnia.Methods 24 female SD rats were divided randomly into 4 groups with 6 rats in each group,i.e.the control group,the model group,the diazepam group and the laser acupoint irradiation group.The model of insomnia rats were established by PCPA abdominal injection and the acupoints of Shenmen（HT7） and Sanyinjiao（SP6） were chosed to be irradiated by He-Ne laser.The change of 5-HT,5-HIAA,DA,NE in the hypothalamus of rats were observed by Fluorescence spectrophotometry.Results After laser acupoint irradiation treatment,the content of 5-HT and 5-HIAA increased obviously in contrast with the model group（P0.01） and the diazepam group（P0.05）,while that of NE and DA decreased obviously in contrast with the model group（P0.05） and the diazepam group（P0.05）.Conclusion The mechanisms of laser acupoint irradiation in treating insomnia may related to its regulation on monoamine neurotransmitter.