蔬菜、水果中16种有机磷和氨基甲酸酯农药残留检测方法研究

Research on detection method of pesticides residues of organic phosphorus and carbamate in vegetables and fruits

目的建立一种快速测定蔬菜、水果中有机磷和氨基甲酸酯16种农药残留的方法。方法应用石英毛细管气相色谱法,利用高灵敏氮磷检测器,用乙腈和丙酮提取样品进行检测。结果该方法检测16种农药残留的线性范围为5.0～1 000.0μg/kg,相关系数≥0.999 0,最低检出浓度为0.1～0.5μg/kg,样品加标回收率为66.0%～84.0%,RSD为4.8%～9.5%。结论该方法能同时测定蔬菜、水果中的有机磷和氨基甲酸酯类农药,具有灵敏度高、分离效果好、回收率高、变异系数小、检出限低的特点。

[Objective]To establish a method for rapidly detecting 16 kinds of pesticides residues of organic phosphorus and carbamate in vegetables and fruits[Methods]The detection was conducted by applying gas chromatography with quartz capillary,by using nitrogen-phosphorus detector with high sensitivity and through extracting sample by acetonitrile and acetone.[Results]the linear ranges of organophosphorus pesticides were 5.0-1 000.0 μg/kg,r≥0.999 0.The lowest determination limit was 0.1-0.5 μg/kg,the recoveries were 66.0%-84.0%,and RSD was 4.8%-9.5% [Conclusion]This method can determine organic phosphorus and carbamate pesticides simultaneously in vegetables and fruits.It has the characteristics of high sensitivity,good separating effect,high recovery rate,small variance coefficient and low determination limit.