2008年-2010年上海市部分伤寒副伤寒沙门菌耐药分析及分子分型

The drug resistance status and molecular typing of S.typhoid and S.paratyphoid strains in Shanghai from 2008-2010

目的:了解上海市2008年-2010年伤寒、副伤寒沙门菌的耐药及分子分型特点,初步掌握伤寒、副伤寒沙门菌的分子流行特点,为今后的防制工作提供科学依据。方法:采用WHO推荐的改良K-B纸片法,对18株伤寒、副伤寒沙门菌进行13种抗生素敏感性试验;运用脉冲场凝胶电泳分型(PFGE)方法对18株伤寒、副伤寒沙门菌进行分子分型及聚类分析。结果:18株伤寒副伤寒沙门菌对利福平100%耐药,对奈啶酸的耐药率为44.4%,其中伤寒沙门菌对奈啶酸的耐药率为37.5%,5株甲型副伤寒沙门菌对奈啶酸100%耐药,乙型副伤寒对奈啶酸无耐药株。所有菌株对其他11种抗生素均敏感;18株伤寒、副伤寒沙门菌共产生16种PGFE带型,有3株甲型副伤寒沙门菌表现为同一PFGE型别。结论:2008年-2010年伤寒、副伤寒沙门菌对抗生素的敏感性较高;甲型副伤寒沙门菌之间有较高的同源性,伤寒沙门菌的PFGE带型比较分散。

Objective：To know antibiotic resistance and molecular types of S. typhoid and S. paratyphoid strains isolated in Shanghai, to master the epidemiological rules and to provide scientific evidences for the future prevention and control. Methods： Modified K - B method recommended by WHO was used for sensitivity test on 18 strains of S. typhi and S. paratyphi to 13 kinds of antibiotics. Pulsed field gel- electrophoresis（PFGE） was used to classify and analyze the 18 strains of S. typhi and S. para- typhi. Results： The drug resistance rate of 1~ S. typhoid and S. paratyphoid strains was 100% for rifampicin and 44.4% for na- lidixic acid, in which the resistance rate to nalidixic acid was 37.5% for S. typhi and 100% for 5 S. paratyphi A strains. All the strains were sensitive to the rest 11 kinds of antibiotics. All strains of S. paratyphi B were not resistant to nalidixic acid. A total of 18 isolates were classified to 16 types by PFGE and only 3 strains of S. typhi A were classified to the same type. Conclusion： S. typhoid and S. paratyphoid isolates from 2008 to 2010 were in high sensitivity to antibiotics. S. paratyphi A strains shared high homology and PFGE types of S. typhoid strains were dispersive.