肺癌患者呼出气冷凝液p16基因甲基化检测

Detection of methylation of the p16 gene in exhaled breath condensate from lung cancer patients

目的:检测肺癌患者呼出气冷凝液中p16基因异常甲基化情况,探讨呼出气冷凝液中p16基因异常甲基化改变作为肺癌临床辅助诊断分子生物学标志物的可能性。方法:利用甲基化特异性聚合酶链反应(MS-PCR)方法,检测20例病理确诊肺癌患者的呼出气冷凝液标本中p16基因的甲基化情况。结果:20例确诊肺癌患者呼出气冷凝液中p16基因异常甲基化阳性例数为8例(40.0%),其中肺鳞癌、腺癌、小细胞癌患者呼出气冷凝液标本的阳性率分别为40.0%,33.3%和50.0%。p16基因的甲基化异常改变与肿瘤的分期、分型无明显相关性。但是呼出气冷凝液中p16基因甲基化水平均为部分甲基化状态。结论:用呼出气冷凝液标本检测甲基化来诊断和筛选肺癌,p16基因可能不是一个理想的候选基因。

Objective：To detect methylation of p16 gene in exhaled breath condensate （EBC）from lung cancer patients, and to assess its potential as a molecular marker for clinical diagnosis of lung cancer. Methods： With methylation - specific PCR （ MS - PCR）, the status of methylation of the p16 gene was investigated in EBC from 20 lung cancer patients. Results： Among these 20 EBC specimens, abnormal methylation of p16 gene was found in 8 cases（40% ）, the positive rates of squamous - cell lung carci- noma, adenocarcinoma and small - cell bung carcinoma were 40.0%, 33.3% and 50.0% respectively. Abnormal methylation of the p16 gene in EBC was not significantly correlated with the clinical stage and pathological type of the tumor. But methylation of p16 gene in the EBC was part - methylation. Conclusion： The method of detecting methylation in EBC to diagnose and screen lung cancer showed that p16 gene may not a suitable candidate gene.