脑脊液S-100b和NSE检测在病毒性脑膜炎诊断中的应用

Application of S-100b and NSE of cerebrospinal fluid in viral encephalitis diagnosis

目的:探讨脑脊液S-100b和NSE水平在病毒性脑膜炎患儿诊断中的临床价值。方法:采用酶联免疫吸附试验(ELISA)检测脑脊液S-100b水平,采用电化学发光法(ECLI)检测NSE的水平,并与对照组进行比较分析。结果:病毒性脑膜炎组(病脑组)脑脊液中急性期S-100b与NSE检测水平分别为(364.8±81.5)ng/L和(12.5±6.1)μg/L,恢复期二项水平分别为(176.4±21.9)ng/L和(5.7±2.8)μg/L,S-100b和NSE水平急性期显著高于其恢复期和对照组(P<0.05);重症组S-100b和NSE的水平分别为(485.1±122.7)ng/L和(17.4±10.6)μg/L,轻症组二项水平分别为(259.0±67.1)ng/L和(9.1±4.3)μg/L,重症组二项指标显著高于轻症组,轻症组二项指标显著高于对照组(P<0.05)。结论:脑脊液S-100b和NSE水平的测定对病毒性脑膜炎患儿的鉴别诊断有重要临床意义。

Objective：To explore the clinical value of detecting cerebrospinal fluid S - 100b and NSE in children with viral en- cephalitis. Methods： The level of S - 100b in CSF was detected by ELISA, the level of NSE was detected with ECLI, and the levels were compared with those of normal children. Results： The levels of S - 100b and NSE in acute stage were （364.8 ＋ 81.5 ） ng/L and （ 12.5 ± 6.1 ） μg/L, while their levels were （ 176.4 ± 21.9） ng/L and （ 5.7 ± 2.8 ） μg/L in restoration stage, the levels of S - 100b andNSE in acute stage were significantly higher than those of the cases in restoration stage and normal chil- dren（P 〈 0.05）. The levels of S - 100b and NSE in severe group were （485.1± 122.7） ng/L and（ 17.4 ± 10.6） μg/L, while their levels were （259.0 ±67.1）ng/L and（9.1 ±4.3） μg/L in mild group. The levels of S - 100b and NSE in severe group were significantly higher than those of the cases in mild group, they in mild cases were significantly higher than those of the cases in normal group（P 〈 0.05）. Conclusion： It is important to detect the levels of S - 100b and NSE for the differential diagnosis of pediartic patients with viral encephalitis.