微柱凝胶法检测不规则抗体的应用与分析

The analysis and application of the micro-column gel test in irregular antibody detection

目的:探讨微柱凝胶法检测不规则抗体在临床上的应用价值,以保证输血安全。方法:利用微柱凝胶法和凝聚胺法对输血前患者进行血清不规则抗体检测,并对阳性标本进行抗体特异性鉴定。结果:在3200例患者中,共检测出不规则抗体阳性标本13例,阳性率为0.41%。其中抗-D抗体3例、抗-E抗体5例、抗-C抗体2例、抗-Ec抗体、抗-e抗体和抗-M抗体各1例。结论:微柱凝胶法检测不规则抗体是一种简单、快捷、敏感性高的方法,对减少溶血性反应和保证输血安全具有重要意义。

Objective： To investigate the value of micro - column gel test in the detection of irregular antibody and to ensure safety of the blood transfusion. Methods： Micro - column gel test and polybrene test were used to detect serum irregular antibody in patients before blood transfusion and identify the specificity of the positive specimens. Results： 13 irregular antibody positive cases were screened in the 3200 patients and the positive rate was 0.41%. Among them, anti - D was 3 cases, anti - E was 5 ca- ses, anti - C was 2 cases, anti - Ec, anti - e and anti - M were all 1 case. Conclusion： The micro - column gel test was a sim- ple, rapid and sensitive method in the irregular antibody screening, which had great significance in avoiding hemolytic transfusion reaction and ensuring the safety of blood transfusions.