检测猪繁殖与呼吸综合征病毒抗体ELISA方法的建立

An Indirect-enzyme linked Immunosorbent Assay for Detection of Antibody to Porcine Reproductive and Respiratory Syndrome Virus

本试验通过差速离心法和非线性蔗糖密度梯度离心法纯化猪繁殖与呼吸综合征病毒 (PRRSV)。经三氯_三氟乙烷处理后 ,作为ELISA试验的标准抗原 ,并建立了检测PRRSV抗体ELISA方法。该方法与IFA、IPMA和国外同类商品化试剂盒进行了对比试验 ,表明该方法具有敏感性高、特异性好的特点 ,是一种快速准确检测PRRSV抗体的方法。间接ELISA方法在敏感性上要优于IFA和IPMA。

In this study,differential centrifugation and discontinuous sucrose density gradient centrifugation were adopted to concentrate and purify porcine reproductive respiratory and syndrome virus(PRRSV),The purified PRRSV which is treated with Trichlor_trifluorethan regard as ELISA_antigen.The indirect ELISA was sensitive and specific,and it was superior to both IPMA and IFA in detecting antibodies in the sensitive.