猪、犬旋毛虫DNA限制性片段长度多态性分析

Identification of 4 Trichinella isolates by Restriction Fragment Length Polymorphisms analysis

以旋毛虫国际标准虫种T .spirlais和T .nativa作对照 ,应用DNA限制性片段长度多态性 (RELP)技术对黑龙江省猪、犬旋毛虫进行虫种鉴定。结果显示 :猪旋毛虫和T .spirlais酶切图谱相同 ;犬旋毛虫和T .nativa酶谱一致。结果揭示 ,黑龙江猪旋毛虫为T .spiralis ,犬旋毛虫为T .nativa。

Trichinella from swine and dog in Hei Longjiang province was identified on the basis of Restriction Fragment Length Polymorphisms(RFLP)analysis,the international standard Trichinella isolates:T.spiralis and T.nativa were used as the control.RFLP analysis of 4 Trichinella isolates indicated that swine isolate and T.spiralis were identical,dog isolate and T.nativa were identical,but patterns of the two groups were different to each other.It is concluded that Trichinella from swine and dog belong to Trichinella spiralis and Trichinella nativa respectively.