甘蔗组织培养配方中不同激素效应的研究

The Effect of Different Hormone in Sugarcane (Saccharum officinarum L. ) Tissue Culture

为防止甘蔗接种物的褐变、坏死而在培养基中加入ＰＶＰ条件下，比较了不同激素对分化苗增殖、分化苗变异率、分化苗的叶绿素含量、促根培养的发根数以及鲜物质与干物质积累的效果．结果表明，较高浓度 ＫＴ和 ６－ ＢＡ ２种外源激素混合使用，不仅有利于促进分化芽增殖，而且也能获得高素质的分化苗．

PVP was added to different media to prevent the tissue from brown-turning. The comparisions were made among the hormone on the planted number, variation rate, root number, fresh and dry weights and chlorophyll content. The result showed that mixture of KT and 6-BA with high concentration not only favored the shoot differentiation but also improved the quality of plantlets.